We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Gene Panel Predicts Disease Progession for Patients with B-cell Lymphoma

By LabMedica International staff writers
Posted on 28 Oct 2019
Cancer researchers developed a 29 gene–based weighted prognostic score for predicting event-free survival and overall survival of patients suffering from diffuse large B-cell lymphoma (DLBCL).

DLBCL is the most common type of non-Hodgkin lymphoma, an aggressive cancer that begins in certain immune system cells and can occur almost anywhere in the body. More...
This cancer occurs primarily in older individuals, with a median age of diagnosis at approximately 70 years of age, although it can also occur in children and young adults in rare cases. An elevated level of circulating cell-free DNA (cfDNA) has been associated with tumor mass and poor prognosis in DLBCL, but the tumor-specific molecular alterations in cfDNA with prognostic significance have remained unclear.

To help clarify this issue, investigators at the University of Chicago Medical Center (IL, USA) studied the association between 5-hydroxymethylcytosines (5hmC), a mark of active demethylation and gene activation, in cfDNA from blood plasma and prognosis in newly diagnosed DLBCL patients.

The investigators emplyed the 5hmC-Seal, a highly sensitive chemical labeling–based sequencing technology, to profile genome-wide 5hmC in cfDNA from blood plasma of 48 patients with newly diagnosed DLBCL. This technology used the T4 bacteriophage beta-glucosyltransferase to transfer an engineered glucose moiety containing an azide group onto the hydroxyl group of 5-hmC. The azide group could be chemically modified with biotin for detection, affinity enrichment, and sequencing of 5-hmC–containing DNA fragments. The 5hmC-Seal technology was shown to be a robust profiling approach for enriching and quantifying 5hmC-modified DNA fragments with as little as one to two nanograms of cfDNA in less than five milliliters of plasma.

The investigators tested the hypothesis that 5hmC profiles in cfDNA at the time of diagnosis reflected the clinical characteristics of DLBCL and were associated with survival. Results obtained during the study enabled the development of a 29 gene–based weighted prognostic score for predicting event-free survival and overall survival.

“Our findings, if validated in a larger independent patient population, could impact the cure rate for DLBCL,” said first author Dr. Brian Chiu, associate professor of epidemiology at the University of Chicago Medical Center. “By identifying those patients who are at high-risk of treatment failure, we can see who may benefit from individualized clinical management or earlier treatment with novel or targeted therapies.”

The study was published in the October 8, 2019, online edition of the journal Blood Advances.

Related Links:
University of Chicago Medical Center


New
Gold Member
Quality Control Material
iPLEX Pro Exome QC Panel
Serological Pipet Controller
PIPETBOY GENIUS
New
Gold Member
Hematology Analyzer
Medonic M32B
New
Gel Cards
DG Gel Cards
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








DIASOURCE (A Biovendor Company)

Channels

Molecular Diagnostics

view channel
Image: A diagnostic test can distinguish patients with head and neck squamous cell carcinoma who can be cured with surgery alone (Photo courtesy of University of Turku)

Novel Diagnostic Tool to Revolutionize Treatment Guidance of Head and Neck Cancer

Head and neck squamous cell carcinoma (HNSCC) is a solid tumor type commonly treated with surgery. However, there has been no clinically available method to determine which patients can be cured with surgery... Read more

Hematology

view channel
Image: The microfluidic device for passive separation of platelet-rich plasma from whole blood (Photo courtesy of University of the Basque Country)

Portable and Disposable Device Obtains Platelet-Rich Plasma Without Complex Equipment

Platelet-rich plasma (PRP) plays a crucial role in regenerative medicine due to its ability to accelerate healing and repair tissue. However, obtaining PRP traditionally requires expensive centrifugation... Read more

Immunology

view channel
Image: The 3D paper-based analytical device has shown high clinical accuracy for adult-onset immunodeficiency (Photo courtesy of National Taiwan University)

Paper-Based Device Accurately Detects Immune Defects in 10 Minutes

Patients with hidden immune defects are especially vulnerable to severe and persistent infections, often due to autoantibodies that block interferon-gamma (IFN-γ), a key molecule in immune defense.... Read more

Microbiology

view channel
Image: The groundbreaking salmonella antimicrobial resistance prediction platform has demonstrated 95% accuracy (Photo courtesy of Yujie You et al., DOI: 10.1016/j.eng.2025.01.013)

New Platform Leverages AI and Quantum Computing to Predict Salmonella Antimicrobial Resistance

Antimicrobial-resistant Salmonella strains are a growing public health concern due to the overuse of antimicrobials and the rise of genetic mutations. Accurate prediction of resistance is crucial for effective... Read more

Technology

view channel
Image: The Check4 gene-detection platform (Photo courtesy of IdentifySensors)

Electronic Biosensors Used to Detect Pathogens Can Rapidly Detect Cancer Cells

A major challenge in healthcare is the early and affordable detection of serious diseases such as cancer. Early diagnosis remains difficult due to the complexity of identifying specific genetic markers... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.