We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us

Download Mobile App





Derived Exosomal Protein Biomarkers in Alzheimer’s Disease Diagnosis

By LabMedica International staff writers
Posted on 04 Aug 2016
Alzheimer’s disease results in brain neuronal plaques composed of amyloid beta peptide (Aβ42) and neurofibrillary tangles composed of phosphorylated tau proteins (P-T181-tau and P-S396-tau). More...
Exosomes are shed by brain neurons, freely cross the blood brain barrier and protect and carry proteins from their cellular origin into plasma.

P-T181-tau and P-S396-tau are present at higher than normal concentrations and Aβ42 at lower than normal concentrations in the cerebrospinal fluid of Alzheimer’s disease (AD) patients. These proteins are not high in plasma samples of AD patients in part due to poor blood brain barrier transport and protease activities.

Scientists at Pan Laboratories (Irvine, CA, USA) and their colleagues validated enzyme-linked immunosorbent assays (ELISA) for Aβ42, P-T181-tau and P-S396-tau, and used them to quantify these proteins in neuron-derived exosomal extracts from normal and AD plasma samples. Plasma samples were obtained from patients with mild cognitive impairment (MCI) and dementia due to Alzheimer’s disease (AD), as well as matched normal controls. Exosomes were precipitated from the plasma samples using the ExoQuick preparation (System Biosciences, Palo Alto, CA, USA).

The team reported that ELISA assays for Aβ42, P-T181-tau and P-S396-tau were reproducible and the Inter-assay Coefficient of Variability (CV) was less than 15%. The sensitivity of the biomarker ELISAs varied from 2 to10 pg/mL. Neuron-specific exosomes were prepared from the plasma of normal controls, MCI and AD patients. The reproducibility of the exosome preparations and biomarker levels were monitored in each ELISA. All biomarkers were elevated in MCI patients and AD patients compared to normal.

The authors concluded that they have validated a reproducible procedure to isolate specific neuron-derived exosomes for quantification of specific protein biomarkers in plasma samples. The concentrations of the biomarkers are high in patients with early dementia and Alzheimer’s disease. This procedure may be useful in the early diagnosis of Alzheimer’s disease. The study was presented at the 68th American Association of Clinical Chemistry (AACC) Annual Scientific Meeting held July 31 to August 4, 2016, in Philadelphia, PA, USA.

Related Links:
Pan Laboratories
System Biosciences
American Association of Clinical Chemistry

Gold Member
Quality Control Material
iPLEX Pro Exome QC Panel
Portable Electronic Pipette
Mini 96
Pipette
Accumax Smart Series
Sample Transportation System
Tempus1800 Necto
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Molecular Diagnostics

view channel
Image: LungCanSeek demonstrated an overall sensitivity of 83.5% and specificity of 90.3% in detecting lung cancer (Photo courtesy of AdobeStock)

New Blood Test Offers Affordable and Accurate Early Detection of Lung Cancer

Lung cancer remains the leading cause of cancer-related deaths worldwide, largely because most cases are diagnosed at advanced stages when treatment options are limited. Early detection significantly improves... Read more

Hematology

view channel
Image: New evidence shows viscoelastic testing can improve assessment of blood clotting during postpartum hemorrhage (Photo courtesy of 123RF)

Viscoelastic Testing Could Improve Treatment of Maternal Hemorrhage

Postpartum hemorrhage, severe bleeding after childbirth, remains one of the leading causes of maternal mortality worldwide, yet many of these deaths are preventable. Standard care can be hindered by delays... Read more

Immunology

view channel
Image: The TriVerity IVD test includes the TriVerity cartridge and the Myrna instrument (Photo courtesy of Inflammatix)

Blood-Based Immune Cell Signatures Could Guide Treatment Decisions for Critically Ill Patients

When a patient enters the emergency department in critical condition, clinicians must rapidly decide whether the patient has an infection, whether it is bacterial or viral, and whether immediate treatment... Read more

Industry

view channel
Image: The collaboration supports clinical validation and regulatory submissions of the new T1D 4-plex assay on Revvity’s GSP instrument (Photo courtesy of Revvity)

Revvity and Sanofi Collaborate on Program to Revolutionize Early Detection of Type 1 Diabetes

Type 1 diabetes (T1D) is a lifelong autoimmune condition in which the immune system destroys the pancreas’s insulin-producing beta cells, leading to dependence on insulin therapy. Early detection is critical... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.