We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

BECKMAN COULTER, INC.

Beckman Coulter develops, manufactures and markets laboratory systems, reagents, centrifugation, lab automation, elec... read more Featured Products: More products

Download Mobile App





Faster Method Diagnoses Pediatric Urinary Tract Infections

By LabMedica International staff writers
Posted on 01 Aug 2022
Print article
Image: The iQ200 series automated urine microscopy analyzer produces shortened TAT with standardized results (Photo courtesy of Beckman Coulter)
Image: The iQ200 series automated urine microscopy analyzer produces shortened TAT with standardized results (Photo courtesy of Beckman Coulter)

Urinary tract infections (UTIs) affect the urinary tract, including the bladder (cystitis), urethra (urethritis) or kidneys (kidney infection). UTIs may be treated with antibiotics, but they're not always needed.

UTIs are common in children and when left untreated can cause acute distress, septic shock, and even kidney damage. The gold standard for diagnosing the condition, urine culture, is slow and labor-intensive for laboratory staff, leading doctors to sometimes prescribe antibiotics inappropriately before getting results, thus contributing to the rise of antibiotic resistance.

Biomedical Scientists at the Nationwide Children's Hospital (Columbus, OH, USA) investigated the association between urinalysis results and those of concurrent urine cultures, and to assess the accuracy of a published prediction model for the results of urine culture in a pediatric population. The study was a retrospective chart review of 5,535 children who previously underwent both urinalysis and urine culture for UTI and carried out at the hospital from January 2019 to December 2020. The study population had a median age of 7 years (mean = 9), was 69% female, and of those with positive urine cultures, 85.2% were female.

Dipstick and microscopic sediment urinalysis were performed on an iQ200 Automated Urine Microscopy Analyzer (Beckman Coulter, Brea, CA, USA). Positive urine culture was defined as ≥50,000 colony-forming units/mL of a urinary pathogen. To evaluate their method's performance in children, the team used it to analyze data from the medical records of the children, they entered their age, sex, risk for UTI, and urinalysis results into their test's algorithm to see how accurately it predicted their urine culture results. Six variables were selected for the prediction model UTOPIA equation: ×1:age UTI risk, ×2:sex, ×3:nitrite, ×4:leukocyte esterase (LE), ×5:white blood cell count (WBC) and ×6:grade of bacteria with ×2-×4 and ×6 being categorical.

The investigators reported that the UTOPIA value exhibited an area under the ROC curve value of 0.825, which is significantly higher (P<0.001) than the individual components for ages 0.546 (0.52-0.57), sex 0.575 (0.56-0.59) nitrite 0.618 (0.60-0.63), LE 0.757 (0.74-0.78), the grade of bacteria 0.664 (0.64-0.69), and the WBC count 0.776 (0.75-0.80). The closer the ROC value was to 1, the more accurate the testing strategy.

Jingcai Wang, MD, PhD, CC(NRCC), SH(ASCP)MLS, a Certified Medical Laboratory Scientist and senior author of the study, said, “The test represents a simple way to predict urine culture results. It can potentially reduce unnecessary urine cultures, save money, and reduce use of unnecessary antibiotics in children.”

The authors concluded that the predictive model UTOPIA value demonstrated good diagnostic performance for predicting urine culture results and possibly reducing unnecessary urine culture and antibiotic use in a retrospective pediatric population. The study was presented on July 27, 2022 at the AACC 2022 meeting held in Chicago, IL, USA.

Related Links:
Nationwide Children's Hospital
Beckman Coulter 

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
One Step HbA1c Measuring System
GREENCARE A1c
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
Gold Member
Reagent Reservoirs
Reagent Reservoirs

Print article

Channels

Clinical Chemistry

view channel
Image: The new versatile assay has the ability to measure both total and bioavailable cortisol from serum (Photo courtesy of Aarhus University)

Highly Reliable Cell-Based Assay Enables Accurate Diagnosis of Endocrine Diseases

The conventional methods for measuring free cortisol, the body's stress hormone, from blood or saliva are quite demanding and require sample processing. The most common method, therefore, involves collecting... Read more

Molecular Diagnostics

view channel
Image: The simple blood test can measure changes in cellular metabolism arising from pancreatic cancer (Photo courtesy of Metabolomycs)

Groundbreaking Blood Test Offers Early Pancreatic Cancer Diagnosis

Pancreatic cancer ranks as the third leading cause of cancer-related deaths, primarily due to its late detection. Early discovery of the disease, while it's still treatable, could significantly impact... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Pathology

view channel
Image: The photoacoustic spectral response sensing instrument is based on low-cost laser diodes (Photo courtesy of Khan et al., doi 10.1117/1.JBO.29.1.017002)

Compact Photoacoustic Sensing Instrument Enhances Biomedical Tissue Diagnosis

The pursuit of precise and efficient diagnostic methods is a top priority in the constantly evolving field of biomedical sciences. A promising development in this area is the photoacoustic (PA) technique.... Read more

Industry

view channel
Image: The companies will develop genetic testing systems based on capillary electrophoresis sequencers (Photo courtesy of 123RF)

Sysmex and Hitachi Collaborate on Development of New Genetic Testing Systems

Sysmex Corporation (Kobe, Japan) and Hitachi High-Tech Corporation (Tokyo, Japan) have entered into a collaboration for the development of genetic testing systems using capillary electrophoresis sequencers... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.