We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
GLOBE SCIENTIFIC, LLC

Randox Laboratories

Provides global diagnostic solutions for hospital laboratories, forensic laboratories, research laboratories, pharmac... read more Featured Products: More products

Download Mobile App





New Biochip Array Developed for ApoE4 Classification

By LabMedica International staff writers
Posted on 04 Aug 2016
Apolipoprotein E (APOE) plays a key role in lipid metabolism and is recognized as one of the most powerful genetic risk factors for dementia and other neurodegenerative diseases. More...
It has become one of the most widely studied gene variants in Alzheimer’s disease and constitutes a major consideration for preventive medicine.

Biochip Array Technology (BAT) enables the determination of multiple analytes from a single sample. This technology has been successfully applied to a new biochip array to directly identify from a plasma sample whether patients are ApoE4 heterozygous, homozygous or null through simultaneous detection of both total ApoE levels and specific ApoE4 levels.

Scientists at Randox Teoranta (Dungloe, Ireland) and their colleagues studied an initial cohort of 272 plasma samples of known genotype, which were used to establish initial assay parameters. ApoE exists in three common isoforms (ApoE2, ApoE3 and ApoE4), which are coded by three co-dominant alleles (e2, e3, e4). As such six common ApoE phenotypes exist within the general population E2/E2, E3/E3, E4/E4 (homozygous) and E2/E3, E2/E4, E3/E4 (heterozygous). A further cohort of 112 plasma samples of unknown genotype was utilized to verify performance characteristics established employing the initial cohort.

The team used a simultaneous chemiluminescent biochip-based sandwich immunoassays for measurement of ApoE4 and total ApoE directly from plasma samples were employed and applied to the Evidence Investigator analyzer (Randox Laboratories, Crumlin, UK; www.randox.com). Genotype concordance was further investigated by genotyping these same 112 plasma samples from circulating cell free DNA (cfDNA) through the use of another biochip array platform, based on a combination of multiplex polymerase chain reaction (PCR) and biochip array hybridization, which allows simultaneous detection of APOE specific single nucleotide polymorphisms (SNPs).

The scientists reported that from the initial cohort of 272 samples with known genotypes, 100% were correctly identified as null, heterozygous or homozygous for ApoE4 by the biochip array. From the additional 112 plasma samples, analyzed using BAT for protein and SNPs detection, 100% concordance was found between both approaches. ROC analysis showed that patient samples could be identified as APOE4 positive or negative with 100% sensitivity and 100% specificity, all in approximately three hours.

The investigators concluded that BAT can be successfully applied to provide a platform to rapidly and accurately detect an individual’s APOE4 status directly from a plasma sample. In combination with medical and family history, medication and lifestyle, this can deliver valuable information for personalized medicine approaches. An individual’s APOE status has been shown to affect pre-symptomatic risk, diagnosis, prognosis, and treatment response for a variety of diseases, in particular Alzheimer’s disease. The study was presented at the 68th American Association of Clinical Chemistry (AACC) Annual Scientific Meeting held July 31 to August 4, 2016, in Philadelphia, PA, USA.

Related Links:
Randox
American Association of Clinical Chemistry

New
Gold Member
Latex Test
SLE-Latex Test
Serological Pipet Controller
PIPETBOY GENIUS
New
Integrated Biochemical & Immunological System
Biolumi CX8
New
PlGF Test
Quidel Triage PlGF Test
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Hematology

view channel
Image: CitoCBC is the world first cartridge-based CBC to be granted CLIA Waived status by FDA (Photo courtesy of CytoChip)

Disposable Cartridge-Based Test Delivers Rapid and Accurate CBC Results

Complete Blood Count (CBC) is one of the most commonly ordered lab tests, crucial for diagnosing diseases, monitoring therapies, and conducting routine health screenings. However, more than 90% of physician... Read more

Immunology

view channel
Image: A simple blood test could replace surgical biopsies for early detecion of heart transplant rejection (Photo courtesy of Shutterstock)

Blood Test Detects Organ Rejection in Heart Transplant Patients

Following a heart transplant, patients are required to undergo surgical biopsies so that physicians can assess the possibility of organ rejection. Rejection happens when the recipient’s immune system identifies... Read more

Pathology

view channel
These images illustrate how precision oncology Organ Chips recapitulate individual patients’ responses to chemotherapy (Photo courtesy of Wyss Institute at Harvard University)

Cancer Chip Accurately Predicts Patient-Specific Chemotherapy Response

Esophageal adenocarcinoma (EAC), one of the two primary types of esophageal cancer, ranks as the sixth leading cause of cancer-related deaths worldwide and currently lacks effective targeted therapies.... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.