Drug Development Cooperation Yields Synthetic Route for Anti-Skin Cancer Compounds

Ingenol mebutate, which is applied topically, can destroy precancerous skin cells with unusual swiftness, while sparing healthy skin cells. The treatment has a direct cancer-cell-killing effect, and also induces an inflammatory reaction. Currently, the compound is extracted inefficiently from the plant Euphorbia peplus.

Investigators at the Danish pharmaceutical company LEO Pharma (Ballerup Denmark), the manufacturer of Picato, recently collaborated with colleagues at The Scripps Research Institute (La Jolla, CA, USA) in an attempt to arrive at method for chemical synthesis of the drug.

They reported in the August 1, 2013, online edition of the journal Science Express that they had succeeded in developing a 14-step synthetic method beginning with inexpensive (+)-3-carene and employing a two-phase design (syntheses of ingenol had been described in the past, but they all required more than 37 steps). This newly described synthesis allowed for the creation of fully synthetic analogs of bioactive ingenol derivatives and provided a strategic blueprint for chemical production.

These results validated the concept of two-phase terpene total synthesis as not only an academic curiosity, but also a viable alternative to isolation or bioengineering for the efficient preparation of polyoxygenated terpenoids at the limits of chemical complexity.

“I think that most organic chemists had considered ingenol beyond the reach of scalable chemical synthesis,” said senior author Dr. Phil S. Baran, professor of chemistry at The Scripps Research Institute. “With this study we rebut that argument conclusively, and there are many other complex natural compounds waiting to be synthesized using a strategy like ours—this is really just a glimpse of the future of chemical synthesis.”

“At the time, the only way to get the product was by a relatively lengthy extraction process from the E. peplus plant,” said Michael Sierra, director of external discovery at LEO Pharma. “We were hoping to get a more efficient synthetic route for production, as well as a method that would allow us to make new derivatives. It is a pretty amazing feat: the total synthesis of ingenol within a year and a half of starting our collaboration. It is great to work with a research group like this.”

Related Links:
Leo Pharma
The Scripps Research Institute