We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




POC Assay Developed for Reliable Blood Grouping

By LabMedica International staff writers
Posted on 07 Apr 2017
Print article
Image: ABO grouping for A, B, AB, and O as indicated by the presence of teal color in the observation zone (red dashed box). Images of multiplexed assays testing (C) ABO/Rh and (D) rare blood systems (Photo courtesy of Third Military Medical University).
Image: ABO grouping for A, B, AB, and O as indicated by the presence of teal color in the observation zone (red dashed box). Images of multiplexed assays testing (C) ABO/Rh and (D) rare blood systems (Photo courtesy of Third Military Medical University).
Rapid and accurate blood grouping plays a critical role in multiple scientific disciplines, particularly in the biological and medical sciences and especially for pregnancy, blood transfusion, and bone marrow transplantation.

A fast, accurate, and versatile paper-based blood test has been developed that could be performed without the need for specialized equipment providing a more cost-effective strategy for blood grouping. The blood typing assay is based on the color change that occurs when a common pH indicator dye reacts with blood.

Medical scientists at the Third Military Medical University, Chongqing, China, assayed 3,550 venous and finger prick blood samples on a paper-based test using bromocresol green. Blood groups were primarily identified by a diagnostic laboratory using the BioVue gel-card assay. The paper-based assay used immobilized antibodies and bromocresol green dye for rapid and reliable blood grouping, where dye-assisted color changes corresponding to distinct blood components provide a visual readout.

ABO antigens and five major Rhesus antigens could be detected within 30 seconds and simultaneous forward and reverse ABO blood grouping using small volumes (100 μL) of whole blood was achieved within two minutes through on-chip plasma separation without centrifugation. A machine-learning method was developed to classify the spectral plots corresponding to dye-based color changes, which enabled reproducible automatic grouping. Using optimized operating parameters, the dye-assisted paper assay exhibited comparable accuracy and reproducibility to the classical gel-card assays in grouping 3,550 human blood samples. When translated to the assembly line and low-cost manufacturing, the proposed approach may be developed into a cost-effective and robust universal blood-grouping platform.

The authors concluded that the assay not only provides a new strategy for blood grouping but can also be used in time- and resource-limited situations, such as war zones, in remote areas, and during emergencies. Characterized by an intensified and streamlined workflow capability, the proposed blood-grouping assay may be further developed into highly compact and fully automatic platforms that are highly efficient and economical, making large-scale manufacturing possible. The study was published on March 15, 2017, in the journal Science Translational Medicine.

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
One Step HbA1c Measuring System
GREENCARE A1c
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
Gold Member
Reagent Reservoirs
Reagent Reservoirs

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.