We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us

AGILENT

Agilent provides laboratories worldwide with instruments, services, consumables, applications and expertise, enabling... read more Featured Products: More products

Download Mobile App




Flow Cytometry Of Reactive T-Cells Differentiates Lymphoproliferative Diseases

By LabMedica International staff writers
Posted on 02 Sep 2022

Classic Hodgkin lymphoma (cHL) is an unusual form of lymphoma characterized by a small number of neoplastic Hodgkin and Reed–Sternberg (HRS) cells in an extensive inflammatory background and about 90% of all Hodgkin lymphomas are this type. More...

In light of the frequent non-neoplastic causes of lymphadenopathy and involvement of sensitive locations, lymph node fine-needle aspiration (FNA) or core needle biopsy (CNB) as minimally invasive procedures are frequently the first alternatives to obtain lymph node tissue to diagnose lymphoproliferative disorders.

Clinical Laboratorians at the Zhejiang University School of Medicine (Hangzhou, China) included in a study cohort consisting of 125 males and 31 females with a male-to-female ratio of 4:1. The patients' ages ranged from 1 to 16, with a median age of 7.7 years. Within this cohort, 25 cases of cHL and 44 cases of reactive lymphoid hyperplasia (RLH) were evaluated for their CD3+CD45RO+T-cell population and CD7 expression on T-cells. Of the reactive cases, 13 were Epstein–Barr virus (EBV) positive.

Lymph node biopsy specimens were obtained from the patients, fixed in 10% neutral buffered formalin, and immediately sent to the pathology laboratory. Samples were prepared for staining with hematoxylin and eosin (HE), periodic acid-Schiff (PAS), and immunohistochemistry (IHC) for histopathology analysis. All immunohistochemistry staining was performed using a two-step technique with the DAKO EnVision HRP System (Agilent Technologies, Santa Clara, CA, USA). Single-cell suspensions from each lymph node were prepared according to a standard protocol. The specimens were analyzed for a range of antigens on a FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA).

The investigators reported that of the suspected lymphoma cases, 55.7% (87/156) were diagnosed with non-Hodgkin lymphoma (NHL) and 16.0% (25/156) with Hodgkin lymphoma based on the histopathological features and immunohistochemical results. The NHL group consisted of 27 cases of T-lymphoblastic lymphoma (T-LBL), 10 cases of B-lymphoblastic lymphoma (B-LBL), 20 cases of Burkitt's lymphoma, nine cases of diffuse large B-cell lymphoma (DLBCL), one follicular lymphoma (FL) case, 17 anaplastic large cell lymphoma (ALCL) cases, one NK case, and two cases of NK/T lymphoma.

The overall concordance of FCI data with the histopathologic results of these cases was 81.4%. A reactive expansion of T-cells with increased expression of CD45RO was present in the reactive infiltrate of cHL (CD45RO/CD3, 67.5%) and Epstein–Barr virus (EBV) infected RLH (62.7%) but not in EBV-negative RLH (28.0%). The mean fluorescence intensity (MFI) of CD7 was higher for cHL and differed significantly from EBV-positive RLH (138.5 versus 63.8). A proposed diagnostic algorithm markedly elevated the overall concordance rate from 81.4% to 97.4%.

The authors concluded that immunophenotyping the reactive infiltrate of lymphoid tissue using flow cytometry is a reliable supplement to histopathology for the rapid diagnosis of pediatric cHL. The study was published on August 21, 2022 in the Journal of Clinical Laboratory Analysis.

 

 


New
Gold Member
Hematology Analyzer
Medonic M32B
Portable Electronic Pipette
Mini 96
New
Sperm Quality Analyis Kit
QwikCheck Beads Precision and Linearity Kit
New
Gel Cards
DG Gel Cards
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Molecular Diagnostics

view channel
Image: Over 100 new epigenetic biomarkers may help predict cardiovascular disease risk (Photo courtesy of 123RF)

Routine Blood Draws Could Detect Epigenetic Biomarkers for Predicting Cardiovascular Disease Risk

Cardiovascular disease is a leading cause of death worldwide, yet predicting individual risk remains a persistent challenge. Traditional risk factors, while useful, do not fully capture biological changes... Read more

Immunology

view channel
Image: The VENTANA HER2 (4B5) test is now CE-IVDR approved (Photo courtesy of Roche)

Companion Diagnostic Test Identifies HER2-Ultralow Breast Cancer and Biliary Tract Cancer Patients

Breast cancer is the most common cancer in Europe, with more than 564,000 new cases and 145,000 deaths annually. Metastatic breast cancer is rising in younger populations and remains the leading cause... Read more

Pathology

view channel
Image: An adult fibrosarcoma case report has shown the importance of early diagnosis and targeted therapy (Photo courtesy of Sultana and Sailaja/Oncoscience)

Accurate Pathological Analysis Improves Treatment Outcomes for Adult Fibrosarcoma

Adult fibrosarcoma is a rare and highly aggressive malignancy that develops in connective tissue and often affects the limbs, trunk, or head and neck region. Diagnosis is complex because tumors can mimic... Read more

Technology

view channel
Image: Conceptual design of the CORAL capsule for microbial sampling in the small intestine (H. Mohammed et al., Device (2025). DOI: 10.1016/j.device.2025.100904)

Coral-Inspired Capsule Samples Hidden Bacteria from Small Intestine

The gut microbiome has been linked to conditions ranging from immune disorders to mental health, yet conventional stool tests often fail to capture bacterial populations in the small intestine.... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.