We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics - An LGC Company

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Pathogen Analyzer Combines Advantages of Antigen and PCR Testing

By LabMedica International staff writers
Posted on 03 Nov 2022
Print article
Image: The Pathogen Analyzer can detect 12 types of viruses in a single sweep (Photo courtesy of Fraunhofer Society)
Image: The Pathogen Analyzer can detect 12 types of viruses in a single sweep (Photo courtesy of Fraunhofer Society)

Rapid antigen tests can quickly provide results at home or at a testing site although the accuracy of these tests leaves a lot to be desired. Protein-based tests, where viral antigens are recognized on the chip, are simply not as accurate as nucleic acid-based tests. In other words: Many infections go undetected and false positive results are also possible. A PCR test is needed to get a reliable result; however, this is both significantly more expensive and more time consuming – it can take up to two days for the result to be available. This dilemma over choosing between speed and accuracy could soon become a thing of the past.

A group of researchers from the Fraunhofer Society (Munich, Germany) has developed a Pathogen Analyzer that combines the advantages of antigen and PCR testing to deliver fast and reliable results. Since the Pathogen Analyzer detects the genetic material of the viruses directly, as in a PCR test, the results are extremely accurate. However, it uses a different method to duplicate the genetic material, which means that the result is available after just 20 to 40 minutes. To do this, the researchers printed multiple small hydrogel droplets onto a test chip, which is similar in size to a rapid antigen test; experts call these droplets “biosensors.”

The sample – which, as with previous tests, is obtained via a nasopharyngeal swab and transferred to a buffer solution – is applied to this chip. The test chip is then heated to 62 degrees Celsius in a compact and mobile analyzer. The buffer solution and the high temperature expose the genetic material of the virus and multiply the nucleic acids for quantitative detection within the biosensors. This reaction takes place at a constant temperature – the heating and cooling of the sample liquid that is biochemically necessary for a PCR test is not required. To receive their personalized test results, patients can connect a smartphone app to the analyzer. A light signal in the analyzer detects the amount of the pathogen’s genetic material and sends the final result directly to the affected person.

Another innovative feature of the Pathogen Analyzer is that it can simultaneously detect 12 types of viruses in a single sweep. Taking a multiplexing approach not only increases the reliability of the results but also makes it possible to detect up to 12 different types of viruses simultaneously using a single sample and a single test chip. In the long term, the test should also function without an analyzer, being fully usable with just a smartphone: Cellphones already come equipped with a light source and camera, and the heating element can be integrated into the test chip itself. Then, the researchers hope, the test could provide fast, inexpensive and reliable results not only in central locations such as stadiums or doctors’ offices but also directly at home – and for a wide range of pathogens.

One of the challenges was to help develop the subsequent manufacturing processes for the test and ensure that they were inexpensive – after all, the test should not cost more than one euro when mass-produced. For the chip itself, the researchers are therefore relying on roll-to-roll processing. The individual sample biosensors can be printed using either a 3D printer or the established screen printing process.

“As we developed the system to be modular, it can be quickly adapted to test for new pathogens,” said Daniel Reibert, a scientist at Fraunhofer IPT. “Each biosensor contains capture molecules that emit fluorescent radiation of a different wavelength when irradiated with light if they have captured the appropriate pathogen. As such, each biosensor is like a small individual test.”

Related Links:
Fraunhofer Society

New
Gold Supplier
STI Real-Time PCR Test
Neisseria Gonorrhoeae/Chlamydia Trachomatis/U.urealyticum Real-Time PCR Kit
New
Silver Supplier
Urinalysis Dipstick Control
Dipper Urinalysis Dipstick Control
New
Rapid Immunological FOB Test
Fecal Occult Blood Test
New
Fecal Occult Blood Test
iFOB Assay

Print article
SUGENTECH INC.

Channels

Clinical Chem.

view channel
Image: ELISA kit for liver-type fatty acid–binding protein (L-FABP). The level of L-FABP present in urine reflects the level of renal tubular dysfunction (Photo courtesy of Sekisui Medical Co)

Urinary Biomarkers Predict Weaning From Acute Dialysis Therapy

Acute kidney injury is associated with a higher risk of chronic kidney disease (CKD), end-stage renal disease, and long-term adverse cardiovascular effects. Critically ill patients with acute kidney injury... Read more

Microbiology

view channel
Image: Ring-form trophozoites of Plasmodium vivax in a thin blood smear (Photo courtesy of Centers for Disease Control and Prevention)

Immune Regulators Predict Severity of Plasmodium vivax Malaria

Cytokines and chemokines are immune response molecules that display diverse functions, such as inflammation and immune regulation. In Plasmodium vivax infections, the uncontrolled production of these molecules... Read more

Pathology

view channel
Image: Breast cancer spread uncovered by new molecular microscopy (Photo courtesy of Wellcome Sanger Institute)

New Molecular Microscopy Tool Uncovers Breast Cancer Spread

Breast cancer commonly starts when cells start to grow uncontrollably, often due to mutations in the cells. Overtime the tumor becomes a patchwork of cells, called cancer clones, each with different mutations.... Read more

Industry

view channel
Image: With Cell IDx’s acquisition, Leica Biosystems will be moving its multiplexing menu forward (Photo courtesy of Leica Biosystems)

Leica Biosystems Acquires Cell IDx, Expanding Offerings in Multiplexed Tissue Profiling

Leica Biosystems, a technology leader in automated staining and brightfield and fluorescent imaging (Nussloch, Germany), has acquired Cell IDx, Inc. (San Diego, CA, USA), which provides multiplex staining... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.