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Multiplex Real-Time PCR Assay Simultaneously Detects and Identifies 9 Parasitic Gastrointestinal Pathogens

By LabMedica International staff writers
Posted on 12 May 2023
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Image: The Allplex GI-Helminth(I) Assay is the first marketed multiplex PCR for helminth diagnosis (Photo courtesy of Seegene)
Image: The Allplex GI-Helminth(I) Assay is the first marketed multiplex PCR for helminth diagnosis (Photo courtesy of Seegene)

The field of parasitology has seen a growing interest in molecular biology. Molecular tools have been extensively used in disciplines such as bacteriology and virology, but the identification of intestinal parasites has been hampered by technical hurdles. Specifically, working with stool samples can be problematic due to the high presence of inhibitors that can disrupt PCR reactions. A novel multiplex PCR assay that allows for the reliable and efficient detection of numerous parasites in a single testing run marks a significant milestone in the field of parasitology.

Seegene, Inc.’s (Seoul, Korea) Allplex GI-Helminth(I) Assay is the first commercially available multiplex PCR assay for the identification of helminths in human stool samples. The assay makes it possible to simultaneously detect and identify nine gastrointestinal parasitic pathogens using real-time PCR. The Allplex GI-Helminth(I) Assay can detect one protozoan and eight helminths, all of which are highly pathogenic parasites. The assay, based on Seegene’s exclusive MuDT technology, provides multiple Ct values for each pathogen in a single channel using real-time PCR instrumentation.

By enabling the simultaneous detection of full range of common intestinal parasites, the informative assay assists in appropriate treatment and management for co-infection. In addition, accurate test results allow for quick and proper treatment. The assay offers a convenient workflow using Seegene’s automated One platform, along with automated data interpretation and LIS interlocking with Seegene Viewer.

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