We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics


Werfen provides diagnostic instruments for critical care and hemostasis to meet the testing needs of medical professi... read more Featured Products: More products

Download Mobile App

Immune Reaction to Gut Bacterium May Trigger Rheumatoid Arthritis

By LabMedica International staff writers
Posted on 31 Oct 2022
Print article
Image: Immune Reaction to Gut Bacterium May Trigger Rheumatoid Arthritis (Photo courtesy of Everyday Health)
Image: Immune Reaction to Gut Bacterium May Trigger Rheumatoid Arthritis (Photo courtesy of Everyday Health)

Rheumatoid arthritis is an autoimmune condition in which the immune system attacks the tissues of the joints, especially in the knees, hands, and wrists. This causes inflammation and painful swelling.

People with rheumatoid arthritis often experience mild symptoms for the first time in their 60s, which then gradually worsen over time. However, antibodies that target the body’s own tissues may circulate in the blood for several years before the onset of rheumatoid arthritis. Prevotella copri (Pc), a gut commensal, has been reported to be an immune relevant organism in individuals with rheumatoid arthritis (RA).

A team of Medical Scientists at the University of Colorado Denver (Aurora, CO, USA) hypothesized immune reactivity to Pc may be found in additional RA populations and potentially in individuals at-risk for developing RA. The study compared 98 patients with established rheumatoid arthritis, and 67 people at high risk of developing rheumatoid arthritis, with equal numbers of matched, healthy controls.

Serum levels of IgA and IgG anti-Pc-p27, an immunogenic Pc protein, were analyzed in study participants at-risk for the development of RA, those who transitioned to RA, in those with early RA (< one year of disease), and in those with established RA, compared to matched controls. Additionally, levels of anti-Pc-p27 antibodies were evaluated in individuals stratified by RA-related autoantibody status.

Serum samples were tested for the following autoantibodies: anti-CCP3 (IgG ELISA; Inova Diagnostics, San Diego, CA, USA), and RF isotypes IgA and IgM by ELISA (Quanta Lite kits, Werfen, Bedford, MA, USA). DNA from buffy coat samples was genotyped for the presence of HLA alleles containing the shared epitope (SE). Participants were tested for IgA and IgG antibody responses to Pc-p27, as determined by ELISA.

The investigators reported that overall, participants with RA had significantly higher levels of IgA anti-Pc-p27 antibodies and trends towards higher levels of IgG anti-Pc-p27 antibodies when compared to their matched controls. When stratified by early versus established RA, early RA participants had median values of IgG anti-Pc-p27 antibodies that were overall higher, whereas median values of IgA anti-Pc-p27 were statistically significantly higher in participants with established RA, compared with their matched controls. In the autoantibody specific analyses, the at-risk population with anti-CCP antibodies, but not Rheumatoid Factor (RF), demonstrated trends towards increased levels of IgG anti-Pc-p27. Additionally, RA participants who were CCP+/RF+ had significantly increased levels of IgA anti-Pc-p27 antibodies and a trend toward levels of IgG anti-Pc-p27 antibodies when compared to their matched controls.

Jennifer A. Seifert, MPH, the corresponding author of the study, said, “Our hope is that these findings can help to further elucidate the complex causative role of bacterial commensals in people who are at risk of developing rheumatoid arthritis and in those with rheumatoid arthritis so that targeted therapies can be developed with the goals of providing better treatment and ultimately, prevention of the disease.”

The authors concluded that at-risk participants and those with early RA had overall higher levels of IgG anti-Pc-p27 antibodies and those with established RA had significantly increased levels of IgA anti-Pc-p27 antibodies. The study was published on October 19, 2022 in the journal Arthritis and Rheumatology.

Related Links:
University of Colorado Denver
Inova Diagnostics

Platinum Supplier
ADAMTS-13 Protease Activity Test
ATS-13 Activity Assay
Gold Supplier
Melanoma Panel
UltraSEEK Melanoma Panel
Ferritin Test
WBC Diffrential System

Print article


Clinical Chemistry

view channel
Image: The new assays are designed to run on the B•R•A•H•M•S KRYPTOR compact PLUS clinical chemistry analyzer (Photo courtesy of Thermo Fisher)

Breakthrough Immunoassays to Aid in Risk Assessment of Preeclampsia

Preeclampsia is a life-threatening blood pressure condition that can arise during pregnancy and the postpartum phase. This severe pregnancy complication is a primary cause of maternal and fetal mortality... Read more

Molecular Diagnostics

view channel
Image: The ClarityDX Prostate test can reduce unnecessary prostate biopsies by up to 35% (Photo courtesy of Nanostics)

Innovative Blood Test to Revolutionize Prostate Cancer Detection and Reduce Invasive Biopsies

One in six men will receive a prostate cancer diagnosis during their lives. Thankfully, if caught early, prostate cancer is highly treatable. However, the existing screening process has its limitations.... Read more


view channel
Image: The latest FDA clearance has finally brought HemoScreen to its full potential as a true POC hematology analyzer (Photo courtesy of PixCell)

True POC Hematology Analyzer with Direct Capillary Sampling Enhances Ease-of-Use and Testing Throughput

An innovative 5-part differential Complete Blood Count (CBC) analyzer with direct capillary sampling capability significantly simplifies blood sampling and minimizes the pre-analytical process.... Read more


view channel
Image: On-chip pretreatment and rapid AST based directly on positive blood cultures (Photo courtesy of Liu Yang)

Integrated Solution for Rapid AST Directly From Positive Blood Cultures to Combat Bloodstream Infection

The presence of living bacteria in the bloodstream, known as bacteremia, can lead to serious conditions like bloodstream infections (BSIs) and sepsis, which can often be fatal. Quickly prescribing the... Read more


view channel
Image: A new microscopy method detects treatment-resistant cancer cells early (Photo courtesy of 123RF)

New Rapid-Live Screening Microscopy Technique Enables Early Detection of Treatment-Resistant Cancer Cells

Chemotherapy serves as an effective tool in the fight against cancer, yet some cancer cells can evade treatment by going into a dormant state known as senescence. These so-called therapy-induced senescent... Read more


view channel
Image: A new electrochemical device can quickly and inexpensively identify people at greatest risk for osteoporosis (Photo courtesy of ACS Central Science, 2023)

Electrochemical Device Identifies People at Higher Risk for Osteoporosis Using Single Blood Drop

With the global increase in life expectancy, the incidence of age-related conditions like osteoporosis is increasing. Osteoporosis, affecting around 200 million individuals worldwide, has a higher incidence... Read more


view channel
Image: AACC Middle East is a two-day conference that brings the latest in laboratory medicine to the Middle East region (Photo courtesy of ADLM)

AACC Middle East 2023 to Explore Latest Trends in Clinical Pathology and Laboratory Medicine

The AACC Middle East Conference and Exposition will be held by the Association for Diagnostics & Laboratory Medicine (ADLM - formerly AACC, Washington, DC, USA) in partnership with Life Dx (Abu Dhabi,... Read more
Copyright © 2000-2023 Globetech Media. All rights reserved.