We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics - An LGC Company

BRAHMS GMBH

BRAHMS GmbH, a Thermo Fisher Scientific company, develops and manufactures diagnostic test procedures for improving t... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Cytokine Signature Identifies COVID-19 Patients With Worst Prognosis

By LabMedica International staff writers
Posted on 27 Apr 2022
Print article
Image: The Ella Automated Immunoassay System (Photo courtesy of R&D Systems)
Image: The Ella Automated Immunoassay System (Photo courtesy of R&D Systems)

An overreaction of the immune system, in which excessive levels of proteins called cytokines produce damaging levels of inflammation, can lead to organ failure and death in COVID-19 patients.

It isn’t known, however, which cytokines drive the process. The ability to measure levels of these cytokines when patients are admitted to the hospital would allow those with the worst prognosis to be identified and their therapy personalized.

Laboratory Medicine specialists at the Azienda Sanitaria Universitaria Friuli Centrale (Udine, Italy) carried out a retrospective study of 415 patients (65.5% male) hospitalized with COVID-19 between May 2020 and March 2021. The cohort included patients with disease of all levels of severity. The patients, who had an average age of 70 years, were classified as having mild/moderate disease or severe/critical disease, according to the World Health Organisation definition. Death occurred in 15.7% of the patients who died in hospital and 23.6% had a negative outcome (orotracheal intubation and/or death).

Serum levels of a large panel of cytokines were measured on admission and compared against outcomes, in combination with other biomarkers such as C-reactive protein (CRP) and mid regional pro-adrenomedullin (MR-proADM). MR-proADM plasma concentrations were measured in an automated Kryptor analyzer, using TRACE technology (Kryptor; BRAHMS, Hennigsdorf, Germany). Cytokines were measured by microfluidic ultrasensitive ELISA using the Protein simple plex technology on an Ella Automated Immunoassay System instrument (R&D Systems, Minneapolis, MN, USA).

The team was able to build a decision tree that allowed them to predict those at risk of a negative outcome, based on the levels of the cytokines and other biomarkers in their blood. This first split patients into two groups, based on their IL-6 levels, before using their levels of IL-10, MR-proADM, sIL2Ra, IP10, and CRP to determine whether they were at risk of a negative outcome.

The analysis also revealed that high levels of interferon (IFN)-γ inducible protein (IP-10) on admission can signal an excessive immune response that may lead to the patient developing lung fibrosis and needing intubation. A further finding was that high levels of IL-6, a pro-inflammatory cytokine, can be accompanied by elevated levels of sIL2Ra and IL-10, which have an anti-inflammatory role. This is important, because in such cases, the immunosuppressive drugs normally used to treat severe COVID could do more harm than good.

Emanuela Sozio, MD, an Infectious Disease specialist and lead author of the study, said, “It is not always possible to determine which COVID-19 patients have the worst prognosis, especially early on. It is becoming increasingly clear, however, that the earlier we treat excessive inflammation, the more likely we are to turn it off quickly and definitively and so avoid irreversible organ damage. Our work may help select patients with worse prognoses that need to be admitted to high dependency units, as well as potentially help personalize their treatment.” The study was presented at the European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), held in Lisbon, Portugal, 23-26 April, 2022.

Related Links:
Azienda Sanitaria Universitaria Friuli Centrale
BRAHMS 
R&D Systems 

Gold Supplier
Saliva Collection Kit
SpeciMAX Stabilized Saliva Collection Kit
New
Homogenizer
DP0150 Pulse 150
New
Washer Dispenser
EL406
New
Urine Analyzer
R-600S

Print article

Channels

Clinical Chem.

view channel
Image: The analysis pipeline used to investigate associations between blood metabolites, later life brain imaging measures, and genetic risk for Alzheimer’s disease (Photo courtesy of University College London)

Lipid Measurements Show Potential as Alzheimer’s Disease Biomarkers

Brain changes accompanying ageing are varied and can include pathologies that lead to cognitive impairment, the commonest of which is Alzheimer’s disease (AD). Identifying blood-based signatures of brain... Read more

Molecular Diagnostics

view channel
Image: Researchers have identified 10 new genes linked with hearing loss (Photo courtesy of 123rf.com)

Newly Identified Genes May Help to Screen and Treat Individuals with Hearing Loss

A massive genome-wide association study (GWAS) identified 10 novel gene loci associated with hearing loss, which may aid in screening and treating the disorder. Hearing loss is one of the top contributors... Read more

Hematology

view channel
Image: The CS-2500 analyzer features pre-analytic sample checks and four detection methods simultaneously on a single platform – coagulation end-point, chromogenic kinetic analysis, turbidimetric immunoassay and automated platelet aggregation (Photo courtesy of Sysmex)

Microvascular/Endothelial Dysfunction Contributes to Post-COVID Syndrome Pathogenesis

Post-COVID syndrome (PCS) or Long-COVID is an increasingly recognized complication of acute SARS-CoV-2 infection, characterized by persistent fatigue, reduced exercise tolerance chest pain, shortness of... Read more

Industry

view channel
Image: Sales of lateral flow assays in clinical testing are expected to register a CAGR of 5% through 2032 (Photo courtesy of Pexels)

Global Lateral Flow Assays Market to Surpass USD 11.5 Billion by 2032 Due to Evolving Applications

The global lateral flow assays market was valued at USD 7.2 billion in 2021 and is projected to register a CAGR of 4.7% during 2022-2032 to surpass USD 11.7 billion by the end of 2032, driven by the growing... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.