We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Pathogenic Yeasts Quantified by Flow Cytometry

By LabMedica International staff writers
Posted on 12 Feb 2013
A flow cytometric, single-stain approach to quantify Candida albicans has been investigated as an alternative to the standard viable plate count.

In clinical settings, viable plate counts (VPC) are used to determine the patient's microbial load, but it is labor intensive, time consuming, and only allows detection of those organisms that readily grow on solid media.

Microbiologists at the University of Antwerp (Belgium) investigated a single-stain, flow cytometric approach for the enumeration of viable C. More...
albicans cells. This single-stain strategy is based on the differences in the fluorescence intensity between viable and nonviable cells. In this study, the cells with an intact membrane are considered alive, while the cells with a damaged membrane are defined as dead.

Cell samples were stained with TO-PRO-3 iodide (TP3; Molecular Probes, OR, USA) which has a peak excitation at 642 nm with emission at 661 nm. All flow cytometry (FCM) studies were performed using a FACSCalibur flow cytometer (BD Biosciences, NJ, USA) equipped with a red diode laser (excitation at 633 nm) and a band pass filter measuring red fluorescence (FL4; range 653–669 nm). Molecular Probes' CountBright absolute counting beads were added to the samples as an internal standard to calculate the exact volume analyzed by the flow cytometer.

The authors concluded that the FCM quantification of viable cells using TP3 is a good alternative for the more time consuming and labor-intensive VPC. In comparison to other FCM techniques, this single-stain procedure is simple to apply and cost-effective since only one dye needs to be added. Moreover, the unique excitation/emission profile of TP3 allows the application of additional dyes to assess other cellular parameters with minimal spectral overlap. The study is planned for publication in print on February 15, 2013, in the Journal of Microbiological Methods.

Related Links:

University of Antwerp
Molecular Probes
BD Biosciences




New
Gold Member
Cardiovascular Risk Test
Metabolic Syndrome Array I & II
3-Part Differential Hematology Analyzer
Swelab Alfa Plus Sampler
New
Clinical Chemistry System
P780
New
Sperm Quality Analyis Kit
QwikCheck Beads Precision and Linearity Kit
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








DIASOURCE (A Biovendor Company)

Channels

Molecular Diagnostics

view channel
Image: A diagnostic test can distinguish patients with head and neck squamous cell carcinoma who can be cured with surgery alone (Photo courtesy of University of Turku)

Novel Diagnostic Tool to Revolutionize Treatment Guidance of Head and Neck Cancer

Head and neck squamous cell carcinoma (HNSCC) is a solid tumor type commonly treated with surgery. However, there has been no clinically available method to determine which patients can be cured with surgery... Read more

Hematology

view channel
Image: The microfluidic device for passive separation of platelet-rich plasma from whole blood (Photo courtesy of University of the Basque Country)

Portable and Disposable Device Obtains Platelet-Rich Plasma Without Complex Equipment

Platelet-rich plasma (PRP) plays a crucial role in regenerative medicine due to its ability to accelerate healing and repair tissue. However, obtaining PRP traditionally requires expensive centrifugation... Read more

Immunology

view channel
Image: The 3D paper-based analytical device has shown high clinical accuracy for adult-onset immunodeficiency (Photo courtesy of National Taiwan University)

Paper-Based Device Accurately Detects Immune Defects in 10 Minutes

Patients with hidden immune defects are especially vulnerable to severe and persistent infections, often due to autoantibodies that block interferon-gamma (IFN-γ), a key molecule in immune defense.... Read more

Technology

view channel
Image: The Check4 gene-detection platform (Photo courtesy of IdentifySensors)

Electronic Biosensors Used to Detect Pathogens Can Rapidly Detect Cancer Cells

A major challenge in healthcare is the early and affordable detection of serious diseases such as cancer. Early diagnosis remains difficult due to the complexity of identifying specific genetic markers... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.