We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics - An LGC Company


Biosynex designs, manufactures and distributes Rapid Diagnostic Tests (RDTs) which, in their professional version, al... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Different Kits and PCR Methods Evaluated for Malaria Diagnosis

By LabMedica International staff writers
Posted on 13 Jul 2022
Print article
Image: The BIOSYNEX AMPLIQUICK Malaria is an in vitro molecular diagnostic test for the detection of the five species of Plasmodium (Photo courtesy of Biosynex)
Image: The BIOSYNEX AMPLIQUICK Malaria is an in vitro molecular diagnostic test for the detection of the five species of Plasmodium (Photo courtesy of Biosynex)

Malaria is an infectious disease considered as one of the biggest causes of mortality in endemic areas. This life-threatening disease needs to be quickly diagnosed and treated. The standard diagnostic tools are thick blood smears microscopy and immuno-chromatographic rapid diagnostic tests.

However, these methods lack sensitivity especially in cases of low parasitaemia and non-falciparum infections. Therefore, the need for more accurate and reliable diagnostic tools, such as real-time polymerase chain reaction based methods which have proven greater sensitivity particularly in the screening of malaria, is pronounced.

Scientists at the Hôpital Bichat - Claude Bernard (Paris, France) conducted a study on 183 blood samples received for expert diagnosis at the French National Malaria Reference Centre (FNMRC). The team assessed sensitivity and specificity of two commercial malaria qPCR kits and two in-house developed qPCRs compared to LAMP.

The samples were tested by four different qPCR methods: the Ampliquick Malaria test (Biosynex SA, Illkirch-Graffenstaden France), the BioEvolution Plasmodium Typage test (Bio-Evolution, Île-de-France, France), the in-house HRM and the in-house TaqMan qPCRs. The specificity and sensitivity of each method and their confidence intervals were determined with the LAMP-based assay Alethia Malaria (Meridian Bioscience, Cincinnati, OH, USA) used as the reference for malaria diagnosis. The accuracy of species diagnosis of the Ampliquick Malaria test and the two in-house qPCRs was also evaluated using the BioEvolution Plasmodium Typage test as the reference method for species identification.

The investigators reported that that when compared to LAMP, a test with excellent diagnostic performances, the two in-house developed qPCRs were the most sensitive (sensitivity at 100% for the in-house TaqMan qPCR and 98.1% for the in-house high resolution melting (HRM) curve analysis qPCR, followed by the two commercial kits: the Biosynex Ampliquick Malaria test (sensitivity at 97.2%) and the BioEvolution Plasmodium Typage (sensitivity at 95.4%). Additionally, with the in-house qPCRs they were able to confirm a Plasmodium falciparum infection in microscopically negative samples that were not detected by commercial qPCR kits. This demonstrated that the var genes of P. falciparum used in these in-house qPCRs are more reliable targets than the 18S sRNA commonly used in most of the developed qPCR methods for malaria diagnosis.

Analysis with the Bio-Evolution kit showed 135 positive and 48 negative samples. Of the 135 positive samples: 73 were identified as P. falciparum, 36 were identified as P. ovale spp, eight were identified as P. vivax, 15 were identified as P. malariae, two mixed infections by P. falciparum and P. malariae and one mixed infection P. falciparum, P. malariae and P. ovale. The 147 isolates tested with LAMP were assessed with the four different qPCR methods. The 43 LAMP negative samples were also negative by all four methods, which afford them all 100% specificity. Of the 104 positive samples, five were not detected by Plasmodium Typage (Bio-Evolution), three by Ampliquick Malaria and two by the HRM-qPCR, but all with the in-house TaqMan-qPCR.

The authors concluded that overall, their results accentuate the role molecular methods could play in the screening of malaria. This may represent a helpful tool for other laboratories looking to implement molecular diagnosis methods in their routine analysis, which could be essential for the detection and treatment of malaria carriers and even for the eradication of this disease. The study was published on June 27, 2022 in the Malaria Journal.

Related Links:
Hôpital Bichat - Claude Bernard 
Biosynex SA 
Meridian Bioscience 

Gold Supplier
Vaginosis Test
Urine Test Strip
HealthMate DA-7
Lab Incubator
Automated Nucleic Acid Extraction Instrument

Print article


Clinical Chem.

view channel
Image: ELISA kit for liver-type fatty acid–binding protein (L-FABP). The level of L-FABP present in urine reflects the level of renal tubular dysfunction (Photo courtesy of Sekisui Medical Co)

Urinary Biomarkers Predict Weaning From Acute Dialysis Therapy

Acute kidney injury is associated with a higher risk of chronic kidney disease (CKD), end-stage renal disease, and long-term adverse cardiovascular effects. Critically ill patients with acute kidney injury... Read more


view channel
Image: Breast cancer spread uncovered by new molecular microscopy (Photo courtesy of Wellcome Sanger Institute)

New Molecular Microscopy Tool Uncovers Breast Cancer Spread

Breast cancer commonly starts when cells start to grow uncontrollably, often due to mutations in the cells. Overtime the tumor becomes a patchwork of cells, called cancer clones, each with different mutations.... Read more


view channel
Image: With Cell IDx’s acquisition, Leica Biosystems will be moving its multiplexing menu forward (Photo courtesy of Leica Biosystems)

Leica Biosystems Acquires Cell IDx, Expanding Offerings in Multiplexed Tissue Profiling

Leica Biosystems, a technology leader in automated staining and brightfield and fluorescent imaging (Nussloch, Germany), has acquired Cell IDx, Inc. (San Diego, CA, USA), which provides multiplex staining... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.