Ultraviolet Surface Excitation Microscopy Emerges as Diagnostic Tool

The approach, which they named microscopy with ultraviolet surface excitation (MUSE), can also generate shape and color-contrast information. MUSE relies on ~280 nm ultraviolet light to restrict the excitation of conventional fluorescent stains to tissue surfaces and it has no significant effects on downstream molecular assays (including fluorescence in situ hybridization and RNA sequencing).

Samples that have been stained with eosin or other standard dyes to highlight important features such as nuclei, cytoplasm and extracellular components produce signals from the UV excitation that are bright enough to be detected by conventional color cameras using sub-second exposure times. The process allows for rapid imaging of large areas and immediate interpretation. MUSE's ability to quickly gather high-resolution images without consuming the tissue is an especially important feature. The technology is being commercialized by MUSE Microscopy Inc (Davis, CA, USA).

Richard Levenson, MD, FCAP, professor and vice chair for strategic technologies in the Department of Pathology and Laboratory Medicine at UC Davis and senior author of the study, said, “MUSE eliminates any need for conventional tissue processing with formalin fixation, paraffin embedding or thin-sectioning. It doesn't require lasers, confocal, multiphoton or optical coherence tomography instrumentation, and the simple technology makes it well suited for deployment wherever biopsies are obtained and evaluated.” The study was published on December 4, 2017, in the journal Nature Biomedical Engineering.

Related Links:
University of California Davis Medical Center
MUSE Microscopy