We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Download Mobile App




Events

15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024
25 Apr 2024 - 27 Apr 2024

Breakthrough Molecular Detection Platform Delivers Best-In-Class Accuracy without Need for Target Amplification

By LabMedica International staff writers
Posted on 18 Oct 2023
Print article
Image: The CRISPR Cascade platform eliminates the need for target amplification (Photo courtesy of VedaBio)
Image: The CRISPR Cascade platform eliminates the need for target amplification (Photo courtesy of VedaBio)

A disruptive new platform that operates with a combination of unprecedented speed and accuracy promises to revolutionize molecular detection and emerge as a new gold standard tool to empower clinical decision-making.

VedaBio (San Diego, CA, USA) has harnessed the immense potential of CRISPR technology to create CRISPR Cascade, a game-changing platform that's the outcome of integrating engineering and biology. The CRISPR Cascade eliminates the requirement for target amplification yet offers the same level of accuracy as PCR tests, delivering analytical results in less than a minute. Unlike traditional DNA/RNA amplification methods like PCR, where increasing the complexity of the assay by multiplexing complicates things, CRISPR Cascade eliminates these constraints. It allows for extremely simple, highly multiplexed tests without sacrificing signal quality or encountering cross-reactivity. The platform offers a blend of high sensitivity, precision, and nearly immediate results, opening up a wide range of new possibilities.

One of the standout features of the CRISPR Cascade is its unprecedented speed and processing power, which facilitate almost instant results best-in-class accuracy. This is made possible by the use of two specific ribonucleoproteins (RNPs): the first, known as RNP1, identifies the target genetic sequence, while the second, RNP2, carries out an additional function, like signal amplification, for example. In contrast to existing DNA amplification methods like PCR, where combining multiple primers can reduce the assay's sensitivity and specificity, CRISPR Cascade can pool different RNP1s, allowing for massive multiplexing without any loss in signal-to-noise ratio (SNR) or cross-reactivity issues. This means that creating new tests or target applications becomes a matter of simple RNP1 adjustments. These modifications are compatible with various types of reporting mechanisms, including fluorescence and colorimetric methods. Thanks to its robust, room-temperature chemistry that is highly compatible with microfluidics, CRISPR Cascade enables almost immediate molecular detection along with significant multiplexing capabilities.

“With the CRISPR Cascade reaction, we have unlocked the true power of CRISPR with a platform that delivers near-instant molecular detection of highly multiplexed analytes with best-in-class accuracy, all without the need for target amplification,” said Anurup Ganguli, PhD, Co-Founder and Chief Executive Officer of VedaBio. “We believe it is poised to emerge as a future gold standard for molecular detection, empowering major advances across multiple applications including research and industrial tools, diagnostics and therapeutics.”

Related Links:
VedaBio 

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Magnetic Bead Separation Modules
MAG and HEATMAG
Complement 3 (C3) Test
GPP-100 C3 Kit
Gold Member
Real-time PCR System
GentierX3 Series

Print article

Channels

Clinical Chemistry

view channel
Image: The 3D printed miniature ionizer is a key component of a mass spectrometer (Photo courtesy of MIT)

3D Printed Point-Of-Care Mass Spectrometer Outperforms State-Of-The-Art Models

Mass spectrometry is a precise technique for identifying the chemical components of a sample and has significant potential for monitoring chronic illness health states, such as measuring hormone levels... Read more

Hematology

view channel
Image: The CAPILLARYS 3 DBS devices have received U.S. FDA 510(k) clearance (Photo courtesy of Sebia)

Next Generation Instrument Screens for Hemoglobin Disorders in Newborns

Hemoglobinopathies, the most widespread inherited conditions globally, affect about 7% of the population as carriers, with 2.7% of newborns being born with these conditions. The spectrum of clinical manifestations... Read more

Immunology

view channel
Image: The groundbreaking treatment approach has shown promise in hard-to-treat cancers (Photo courtesy of 123RF)

Genetic Testing Combined With Personalized Drug Screening On Tumor Samples to Revolutionize Cancer Treatment

Cancer treatment typically adheres to a standard of care—established, statistically validated regimens that are effective for the majority of patients. However, the disease’s inherent variability means... Read more

Pathology

view channel
Image: ‘Virtual biopsy’ allows clinicians to analyze skin noninvasively (Photo courtesy of Stanford Medicine)

Virtual Skin Biopsy Determines Presence of Cancerous Cells

When dermatologists spot an unusual mark on a patient's skin, they face a choice: monitor it for some time or remove it for biopsy. Similarly, when removing breast tumors, surgeons must send excised tissues... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.