We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Multiplex Molecular Tests Detect Seven Intestinal Parasites

By LabMedica International staff writers
Posted on 28 Mar 2011
Polymerase chain reaction (PCR) assays for intestinal parasites can be used on fecal DNA samples for enhanced detection of pathogenic organisms.

A multiplex PCR-based assay for the ova and parasite stool examination is now available and the molecular technology is comparable with microscopy and copro-antigen detection systems. More...


Scientists at the University of Virginia (Charlottesville, VA, USA), adapted several existing real time PCR assays into a high throughput protocol for the major intestinal parasites. Fecal DNA specimens were obtained from 192 preschool-age or younger children from Bangladesh and 190 DNA specimens were obtained from the Leiden University Medical Center (Leiden, Netherlands). Positive control materials were included in this study. The assay involves two multiplex PCR reactions, one with specific primers for the protozoa and one with specific primers for the helminths, after which PCR products are hybridized to beads linked to internal oligonucleotide probes and detected on a Luminex platform (Luminex Corporation, Austin, TX, USA).

The Luminex protozoa assay showed a low limit of detection of 1,000 Giardia lamblia cysts, 100 Cryptosporidium parvum oocysts, and 10 Entamoeba histolytica trophozoites in 200 mg of a stool specimen. The Luminex helminth assay could detect Ancylostoma duodenale, Ascaris lumbricoides, Necator americanus, and Strongyloides stericoralis in very low concentrations. When compared with the parent multiplex real-time PCR assays, this multiplex PCR-bead assay afforded between 83% and 100% sensitivity and specificity on 319 clinical specimens.

The authors concluded that the multiplex PCR-bead protocol provides an alternative high throughput molecular diagnostic platform for specific and sensitive detection of several major intestinal parasites and is a potential alternative to microscopy for equipped laboratories. The study was published in February 2011, in the American Journal of Tropical Medicine and Hygiene.

Related Links:
University of Virginia
Leiden University Medical Center
Luminex Corporation



New
Gold Member
Serological Pipets
INTEGRA Serological Pipets
Serological Pipet Controller
PIPETBOY GENIUS
New
Anterior Nasal Specimen Collection Swabs
53-1195-TFS, 53-0100-TFS, 53-0101-TFS, 53-4582-TFS
New
Sperm Quality Analyis Kit
QwikCheck Beads Precision and Linearity Kit
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








DIASOURCE (A Biovendor Company)

Channels

Hematology

view channel
Image: The microfluidic device for passive separation of platelet-rich plasma from whole blood (Photo courtesy of University of the Basque Country)

Portable and Disposable Device Obtains Platelet-Rich Plasma Without Complex Equipment

Platelet-rich plasma (PRP) plays a crucial role in regenerative medicine due to its ability to accelerate healing and repair tissue. However, obtaining PRP traditionally requires expensive centrifugation... Read more

Immunology

view channel
Image: The 3D paper-based analytical device has shown high clinical accuracy for adult-onset immunodeficiency (Photo courtesy of National Taiwan University)

Paper-Based Device Accurately Detects Immune Defects in 10 Minutes

Patients with hidden immune defects are especially vulnerable to severe and persistent infections, often due to autoantibodies that block interferon-gamma (IFN-γ), a key molecule in immune defense.... Read more

Pathology

view channel
Image: Researchers have developed a novel method to analyze tumor growth rates (Photo courtesy of Adobe Stock)

Novel Method To Analyze Tumor Growth Rates Helps Tracks Progression Between Diagnosis and Surgery

Patients diagnosed with breast cancer often worry about how quickly their tumors grow while they wait for surgery, and whether delays in treatment might allow the disease to spread beyond the point of cure.... Read more

Technology

view channel
Image: The newly designed ExoPatch successfully distinguished melanoma from healthy skin in mice (Photo courtesy of Jeremy Little/Michigan Engineering)

Microneedle Skin Patch Detects Melanoma Without Biopsy or Blood Draw

Melanoma, the most aggressive form of skin cancer, currently requires patients, especially those with fair skin and moles, to undergo regular doctor visits and biopsies every six months to determine if... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.