We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us

Download Mobile App

Serological Markers of RA Determined in Patients with aβ2GPI-IgA

By LabMedica International staff writers
Posted on 14 Jun 2022
Print article
Image: The gold standard for the determination of antinuclear antibodies (ANA) is the indirect immunofluorescence test (IIFT) with human epithelial cells (HEp-2), which is known for its high sensitivity and specificity (Photo courtesy of Euroimmun)
Image: The gold standard for the determination of antinuclear antibodies (ANA) is the indirect immunofluorescence test (IIFT) with human epithelial cells (HEp-2), which is known for its high sensitivity and specificity (Photo courtesy of Euroimmun)

Rheumatoid arthritis (RA) is a chronic and systemic autoimmune disease. It is characterized by synovial inflammation that can lead to irreversible joint damage and disability when not managed. Biomarkers of RA available for routine clinical use are rheumatoid factors (RF) and anti-cyclic citrullinated peptides (CCP-Ab).

Beta 2 Glycoprotein I (β2GPI) is a plasma protein and plays a key role in hemostasis, homeostasis, and immunity. Antibodies against β2GPI (aβ2GPI) have been associated with antiphospholipid syndrome (APS) pathogenicity. Common links between RA and APS have been reported. Glycosylation is implicated in the synthesis of highly pathogenic forms of antibodies to citrullinated proteins. Moreover, dysregulation of glycosylation could also generate antibodies of APS.

Immunologists at the Farhat-Hached University Hospital (Sousse, Tunisia) conducted a retrospective study that included sera of 67 patients with anti-β2 glycoprotein I antibodies (aβ2GPI) of IgA isotype. Ninety healthy blood donors (HBD) were used as a control group. The serum samples were evaluated for aCL-IgG, IgA, and IgM using a commercial enzyme-linked immunosorbent assay (ELISA; Orgentec Diagnostika, Mainz, Germany). Results were expressed as arbitrary units with a cutoff for positivity of 10 U/mL for IgA and IgG and 7 U/mL for IgM.

The determination of aβ2GPI IgG, IgA, and IgM was carried out with an Orgentec Diagnostika commercial ELISA, using a purified human β2GPI. The results were expressed as arbitrary units with a cutoff for positivity of 8 U/mL. Antinuclear antibodies screening was performed by the indirect immunofluorescence technique using human epithelial type 2 (HEp-2) as a substrate (Euroimmun, Hamburg, Germany). Detection of ANA at a dilution superior to or equal to 1:80 was considered a positive result.

The scientists reported that 17 patients and eight HBD had CCP-Ab and/or RF (25.4% versus 8.9%, odds ratio = 3.5). The frequency of CCP-Ab was significantly higher in patients than in healthy subjects (14.9% versus 3.3%). IgA isotype of RF was significantly higher in patients than in controls (7.5% versus 0%). In male patients, CCP-Ab and/or RF were more frequent than in healthy male subjects (37.5% versus 11.8%). In patients, no correlation was found between the levels of aβ2GPI-IgA and CCP-Ab. There was no correlation between the level aβ2GPI-IgA and the level of the isotypes of RF (IgG, IgA, and IgM).

The authors concluded that a high frequency of autoantibodies of RA in patients with aβ2GPI-IgA, suggesting that these patients could be at higher risk of developing RA. Following the clinical features and serological markers of these patients over the years could confirm this data. The study was published on June 6, 2022 in the Journal of Clinical Laboratory Analysis.

Related Links:
Farhat-Hached University Hospital
Orgentec Diagnostika 

Platinum Supplier
ADAMTS-13 Protease Activity Test
ATS-13 Activity Assay
Gold Supplier
Pipette Controller
Sapphire MaxiPette
POCT Fluorescent Immunoassay Analyzer
Fecal Occult Blood Test
Seracult Triple Slide FOBT

Print article


Clinical Chemistry

view channel
Image: A module with eight micro-devices, complete with microfluidic channels and drive motors (Photo courtesy of U.S Department of Energy)

Highly Sensitive pH Sensor to Aid Detection of Cancers and Vector-Borne Viruses

Understanding the acidity or alkalinity of substances through pH measurement is crucial in many fields, from environmental monitoring to healthcare product safety. In many cases, these measurements must... Read more

Molecular Diagnostics

view channel
Image: Newly identified biomarkers may detect early cognitive decline via a blood test (Photo courtesy of 123RF)

Blood Tests to Enable Early Identification of Cognitive Decline and Personalized Treatments

Some individuals who have endured extreme stressors like psychiatric disorders or childhood abuse and neglect can experience a variety of health issues later in life, such as depression, anxiety, and cardiovascular... Read more


view channel
Image: The QScout hematology analyzer has received US FDA 510(k) clearance (Photo courtesy of Ad Astra Diagnostics)

First Rapid-Result Hematology Analyzer Reports Measures of Infection and Severity at POC

Sepsis, a critical medical condition that arises as an extreme response to infection, poses a significant health threat. It occurs when an infection triggers a widespread inflammatory response in the body.... Read more


view channel
Image: Current testing methods for antibiotic susceptibility rely on growing bacterial colonies in the presence of antibiotics (Photo courtesy of 123RF)

Rapid Antimicrobial Susceptibility Test Returns Results within 30 Minutes

In 2019, antimicrobial resistance (AMR) was responsible for the deaths of approximately 1.3 million individuals. The conventional approach for testing antimicrobial susceptibility involves cultivating... Read more


view channel
Image: AI methods used in satellite imaging can help researchers analyze tumor images (Photo courtesy of Karolinska Institutet)

AI Approach Combines Satellite Imaging and Ecology Techniques for Analysis of Tumor Tissue

Advancements in tumor imaging technology have significantly enhanced our ability to observe the minute details of tumors, but this also brings the challenge of interpreting vast amounts of data generated... Read more


view channel
Image: Made-to-order diagnostic tests may soon be on the horizon (Photo courtesy of McGill University)

Breakthrough in Diagnostic Technology Could Make On-The-Spot Testing Widely Accessible

Home testing gained significant importance during the COVID-19 pandemic, yet the availability of rapid tests is limited, and most of them can only drive one liquid across the strip, leading to continued... Read more


view channel
Image: The acquisition significantly expands Medix Biochemica’s portfolio of IVD raw materials (Photo courtesy of ViroStat)

Medix Biochemica Acquires US-Based ViroStat to Expand Infectious Diseases Antibody Offering

Medix Biochemica (Espoo, Finland), a supplier of critical raw materials to the in vitro diagnostics (IVD) industry, has acquired ViroStat LLC (Portland, ME, USA), a provider of infectious disease antibodies... Read more
Copyright © 2000-2023 Globetech Media. All rights reserved.