Circulating T Cell-Monocyte Complexes Indicate Immune Perturbations

For some latent TB and TB negative subjects, leukapheresis was performed instead of a whole blood donation in order to increase the number of peripheral blood mononuclear cells (PBMC) obtained. Laboratory parameters such as platelet and leukocyte counts, hematocrit, hemoglobin, AST, ALT and if applicable an ultrasound examination of the chest and abdomen or an X-ray were used to further diagnose patients with either dengue fever (DF) or dengue hemorrhagic fever (DHF).

Latent TBI status was confirmed in subjects by a positive IFN-γ release assay (IGRA), QuantiFERON-TB Gold In-Tube or T-SPOT.TB and the absence of clinical and radiographic signs of active TB. TB uninfected control subjects were confirmed as IGRA negative. Magnetic red blood cell (RBC) depletion was performed using the EasySep RBC depletion kit. PBMC were processed and acquisition was performed on a BD LSR-II cell analyzer or on their BD FACSAria III cell sorter.

The team showed that cell:cell doublets pairing a T cell and a monocyte can be directly isolated from human blood, and high resolution microscopy shows polarized distribution of LFA1/ICAM1 in many doublets, suggesting in vivo formation. Intriguingly, T cell-monocyte complex frequency and phenotype fluctuate with the onset of immune perturbations such as infection or immunization, reflecting expected polarization of immune responses. Bjoern Peters, PhD, a professor in the Vaccine Discovery Division at LJI and senior author of the study, said, “It's an important lesson learned by studying these cells and how they communicate, we might learn more about the immune system.” The study was published on June 25, 2019, in the journal eLife.

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