We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us

BIO-RAD LABORATORIES

Provides full range of instrumentation, reagent kits, software and quality control systems to clinical laboratories. ... read more Featured Products: More products

Download Mobile App




Anti-Double Stranded DNA Antibodies Diagnose Systemic Lupus Erythematosus

By LabMedica International staff writers
Posted on 13 Jul 2022

Anti-double-stranded deoxyribonucleic acid antibodies (anti-dsDNA Abs) embrace antibodies with a wide spectrum of fine molecular specificities that are produced persistently in the context of true autoimmunity or transiently in context of infections or other clinical conditions. More...

Numerous studies have yielded insight into specific aspects of anti-dsDNA Abs, such as their genetics, immunogens and targets, as well as their diagnostic and pathogenic impact, particularly in Systemic Lupus Erythematosus (SLE). The clinical significance of anti-dsDNA Abs largely depends on the principle of the assay employed and the analytical variables of the methods used to quantify and characterize them.

A team of Immunologists at the University Hospital Mohamed VI (Marrakech, Morocco) and their colleagues carried out a cross-sectional study on 113 adults and pediatric patients presenting various clinical conditions that included 53 SLE, other systemic autoimmune rheumatic diseases (OSARD) including 23 rheumatoid arthritis, nine Sjögren syndrome, eight mixed connective tissue disease (MCTD) five systemic sclerosis (SSc, inflammatory myositis (IM), and 10 non-auto-immune clinical conditions (NAICC), including five patients with inflammatory neurologic diseases and five cases of non-lupus glomerulonephritis. The mean age of patients was 39.9 ± 15 years (range, 11–85 years), and they were predominantly females (female to male sex ratio = 4.94).

Blood samples collected from the patients and serum samples were tested for anti-dsDNA Abs using the ELISA Kallestad Anti-dsDNA EIA, IgG conjugate (Bio-Rad Laboratories, Hercules, CA, USA) combined with and Crithidia luciliae fluorescence test (CLIFT, Kallestad, Bio-Rad), threshold: 1:10). Screening for ANA was performed by the IIF method using a Hep-2 cell substrate (Kallestad, Bio-Rad), threshold: 1:160 for patients older than 18 and 1:80 for those younger than 18).

The investigators reported that overall, 42.4% (n = 48), 27.4% (n = 31), and 78.8% (n = 89) of patients with selected clinical conditions were positive for DNA-ELISA, DNA-CLIFT, and ANA-IIFA anti-dsDNA Ab screening, respectively The proportion of ELISA-positive patients with SLE was more than that of CLIFT-positive ones (77.3% versus 54.7%). The DNA-ELISA test was positive for 48 patients (42.4%), among whom 34 patients had titers higher than 50 IU/mL, 16 patients had titers varying between 30 and 50 IU/mL, whereas 32 patients had equivocal titers (15–30 IU/mL), and 31 patients were negative. ANA were positive for 78.7% of patients, including 48 patients (90.5%) with SLE. Among the 50 patients positive for DNA-ELISA, 31 (62%) were also positive for CLIFT, and these patient were significantly associated with high titers of DNA-ELISA.

The authors concluded that a diagnostic strategy based on the detection and quantification of anti-dsDNA Abs by ELISA, followed if positive by CLIFT and ANA-IIF screening. High titers of anti-DNA Abs on ELISA associated with positive CLIFT are almost relevant, especially when associated with a homogeneous ANA-IIF pattern, taking into consideration the clinical criteria of SLE. The study was published on June 9, 2022 in the journal Practical Laboratory Medicine.

Related Links:
University Hospital Mohamed VI 
Bio-Rad Laboratories 


New
Gold Member
Hematology Analyzer
Medonic M32B
POC Helicobacter Pylori Test Kit
Hepy Urease Test
New
Automatic Hematology Analyzer
DH-800 Series
New
Clinical Chemistry System
P780
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Immunology

view channel
Image: The test could streamline clinical decision-making by identifying ideal candidates for immunotherapy upfront (Xiao, Y. et al. Cancer Biology & Medicine July 2025, 20250038)

Blood Test Predicts Immunotherapy Efficacy in Triple-Negative Breast Cancer

Triple-negative breast cancer (TNBC) is an aggressive subtype lacking targeted therapies, making immunotherapy a promising yet unpredictable option. Current biomarkers such as PD-L1 expression or tumor... Read more

Microbiology

view channel
Image: New diagnostics could predict a woman’s risk of a common sexually transmitted infection (Photo courtesy of 123RF)

New Markers Could Predict Risk of Severe Chlamydia Infection

Chlamydia trachomatis is a common sexually transmitted infection that can cause pelvic inflammatory disease, infertility, and other reproductive complications when it spreads to the upper genital tract.... Read more

Technology

view channel
Image: The sensor can help diagnose diabetes and prediabetes on-site in a few minutes using just a breath sample (Photo courtesy of Larry Cheng/Penn State)

Graphene-Based Sensor Uses Breath Sample to Identify Diabetes and Prediabetes in Minutes

About 37 million U.S. adults live with diabetes, and one in five is unaware of their condition. Diagnosing diabetes often requires blood draws or lab visits, which are costly and inconvenient.... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.