Methods Developed to Generate Normal Stem Cells from Patients with Mitochondrial Defects

Clinical manifestations vary based on mutation type and the relative levels of mutant and normal mtDNA within each cell.

Investigators at the Salk Institute for Biological Studies (La Jolla, CA, USA) first described the situation of patients having both normal and mutated mtDNA. Skin cells from these patients could be turned into a population of stem cells, some with normal mtDNA and some with mutated mtDNA. It was then a simple matter to clone only the cells with normal mtDNA to form a population of normal pluripotent stem cells.

The other case centered on patients with few, if any cells with normal mtDNA. To solve this problem the investigators removed the nuclei of the patient's skin cells, which contain most of their genes, and transplanted them into donor egg cells with healthy mitochondria. The new egg cells were then used to generate healthy pluripotent stem cells.

Results published in the July 15, 2015, online edition of the journal Nature revealed that both reprogramming approaches offered complementary strategies for derivation of pluripotent stem cells containing exclusively normal mtDNA.

"Right now, there are no cures for mitochondrial diseases," said senior author Dr. Juan Carlos Izpisua Belmonte, professor of genetics at the Salk Institute. "Very recently, we have developed ways to prevent these diseases, so it was natural to next ask how we could treat them."

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