Highly Aligned Nanofibers Developed for Tissue Engineering Applications

Such cells adopt growth characteristics, morphological appearance, and biological responses that are not expressed when the same cells are maintained on artificial substrata (plastic, glass), even if coated with isolated constituents of ECM such as purified collagen or glycoproteins.

Investigators at Michigan Technological University (Houghton, USA) used synthetic nanogratings (130 nanometers in depth) to direct the growth of human dermal fibroblasts for up to eight weeks, resulting in a uniform 70 micrometer-thick fibroblast cell sheet with highly aligned cells and ECM nanofibers. A natural ECM scaffolding with uniformly aligned nanofibers about 78 nanometers in diameter was generated after removing the cellular components from the fibroblast sheet.

In a report published in the January 29, 2014, online edition of the journal Advanced Functional Materials the investigators demonstrated the excellent capacity of the ECM-scaffolding in directing and supporting alignment and proliferation of reseeded human mesenchymal stem cells (hMSCs) along the underlying fibers. Furthermore, the aligned ECM scaffolding induced a significantly lower immune response compared to its unaligned counterpart, as detected by proinflammatory cytokines secreted from macrophages.

“The cells did the work,” said senior author Dr. Feng Zhao, assistant professor of biomedical engineering at Michigan Technological University. “The material they made is quite uniform, and of course it is completely biological. We think this has great potential. I think we could use this to engineer softer tissues, like skin, blood vessels, and muscle.”

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Michigan Technological University