Small-Scale Incubator Microscope Designed to Study Cells in Time Lapse

It is ideally suited for time-lapse study over a number of weeks and for automatic observation of cell cultures. The incubator microscope is no bigger than a soda can, and costs 30 times less than purchasing an incubator and a microscope separately.

Similar to humans, cells require nutrients to survive. Cultivating human and animal cells requires parameters such as temperature and humidity to be specified with absolute precision and maintained at an even level over long periods of time. Time-lapse observation over a period of some weeks can be particularly beneficial, since a lot occurs in that time in terms of cell reproduction and differentiation. Until now, the typical way to make these sorts of observations has been to use small incubators in combination with traditional microscopes. This takes up about one square meter of space, making operating several such systems alongside each other an inefficient process. There is a need for innovative solutions that will substantially reduce the space needed and the costs involved without compromising the quality of the cultivation and of the microscope images recorded.

The small-scale incubator microscope system can be used for time-lapse observation of cell cultures as well as to collect fluorescent images at different wavelengths. It includes a small incubation chamber and control electronics to provide defined cell culture parameters. Cells grow on the floor of the miniaturized incubation chamber on a thin, replaceable glass plate and are supplied with a constant stream of nutrients. The only parameters that need to be kept constant within the incubator are the temperature and the nutrient supply flow rate. The small-scale incubator microscope allows for many units to be operated in parallel in a very compact space. Moreover, in spite of its space-saving design, the system generates images that are almost as good as those of the bigger microscopes.

Prototype versions are now in use in a range of research projects. “The system is stable and can be used for time-lapse observation spanning several weeks,” commented Dr. Thomas Velten, head of the biomedical microsystems department at the Fraunhofer Institute for Biomedical Engineering IBMT (Berlin, Germany). The device continuously gathers data and saves them to a computer. Images can be accessed at any time and analyzed using the appropriate image processing software.

“Our customers get a biomedical analysis tool of the highest quality—well priced, space-saving, and tailored to their needs,” concluded Dr. Velten. The incubator microscope is suited to a wide variety of applications, for instance examining the reaction of cells to nanoparticles or toxic agents in the environment. Another current application is stem cell research. “The system is compact, mobile, extremely efficient, and fully automatic in operation.”

Related Links:
The Fraunhofer Institute for Biomedical Engineering IBMT