We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
Sekisui Diagnostics

Download Mobile App




Automated Microfluidic Device Simultaneously Detects HIV RNA and Antibodies

By LabMedica International staff writers
Posted on 27 Mar 2016
Diagnostic laboratories now have available a fully automated molecular testing device that simultaneously detects host anti-HIV antibodies and viral RNA in a single specimen of saliva or blood. More...


In developing the system, Rheonix Inc. (Ithaca, NY, USA) optimized a commercially available HIV serologic assay to run within a microfluidic device into which was also incorporated an isothermal LAMP assay to detect the presence of viral RNA. LAMP (Loop-mediated isothermal AMPlification) is highly regarded for its rapidity, sensitivity, and ability to amplify both DNA and RNA.

The finalized system consisted of a disposable injection molded and film-laminated microfluidic CARD (Chemistry and Reagent Device) disposable device and a portable, software controlled instrument, which together automatically performed all steps of both assays without any user intervention after the initial loading of samples and reagents.

The microfluidic CARD cartridge had multiple microchannels, valves, pumps, and reservoirs, which performed the immunoassay, isolated viral RNA for detection by magnetic bead based purification, and Reverse Transcriptase loop-mediated isothermal amplification (RT-LAMP). The microfluidic system was able to detect host anti-HIV antibodies and viral RNA simultaneously in up to four blood or saliva samples.

The system was shown to detect acute HIV infection and allow immediate confirmation of a seropositive screening result by detection of HIV RNA. It also addressed the well-known "seroconversion window" during early HIV infection when antibodies are not yet detectable and viral loads are at their highest.

“Our technology meets the needs of settings with limited resources. The assays are rapid, sensitive, specific, easy to perform, and inexpensive,” said Dr. Greg Galvin, CEO of Rheonix. “This combined HIV screening and confirmatory test will have a significant commercial and social impact that we’re excited to see materialize.”

The assay device was described in detail in the January 31, 2016, online edition of the Journal of AIDS & Clinical Research.

Related Links:

Rheonix Inc.



Platinum Member
Automated Coagulation Analyzer
Hemolumi H6
Gold Member
Automatic Hematology Analyzer
CF9600
New
Slide Scanner System
NanoZoomer S540MD
HPV Test
Allplex HPV28 Detection
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Clinical Chemistry

view channel
Image Credit: 123RF

Screening After Pneumococcal Disease May Reveal Undiagnosed Blood Cancer or Immune Disorders

Severe pneumococcal disease requiring hospitalization often presents as pneumonia, particularly in older adults and people with cancer or compromised immune function. Because M protein testing and antibody... Read more

Microbiology

view channel
Image Credit: 123RF

FDA-Cleared Multiplex PCR Test Detects 13 Respiratory Pathogens in a Single Sample

Respiratory tract infections can be difficult to distinguish at presentation because many cause overlapping, nonspecific symptoms and are initially grouped as influenza-like illnesses. Causes span a range... Read more

Industry

view channel
Image

Collaboration Combines AI Cognitive Assessment and RNA Blood Testing for Earlier Alzheimer’s Detection

Alzheimer’s disease is often identified only after substantial neurodegeneration, partly because current diagnostic pathways are fragmented and difficult to scale. As treatment shifts toward earlier intervention,... Read more
Copyright © 2000-2026 Globetech Media. All rights reserved.