We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App





Automated PCR Home-Test Device Accurately Detects Covid-19 in Saliva

By LabMedica International staff writers
Posted on 04 Aug 2022
Print article
Image: The SLIDE device analyzes saliva and could help make COVID-19 testing quicker and easier (Photo courtesy of Dr. Weihua Guan, Pennsylvania State University)
Image: The SLIDE device analyzes saliva and could help make COVID-19 testing quicker and easier (Photo courtesy of Dr. Weihua Guan, Pennsylvania State University)

A recently developed saliva-based Covid-19 home test combines the simplicity and speed of rapid antigen tests with the accuracy of the gold-standard RT-PCR method.

Rapid antigen tests for the SARS-CoV-2 (Covid-19) antigen from nasal samples provide a fast (15 minutes) result. However, the sensitivity of antigen tests is 30 to 40% lower than nucleic acid testing (PCR), which could miss a significant portion of infected patients.

With the problem of low sensitivity in mind, investigators at Pennsylvania State University (University Park, PA, USA) developed a fully integrated SARS-CoV-2 reverse transcription loop-mediated isothermal amplification (RT-LAMP) device (SLIDE) using a self-collected saliva sample. The five distinct modules of the SLIDE platform conducted all of the steps needed for RT-LAMP: heating the sample, mixing it with RT-LAMP reagents, carrying out the reaction, detecting how much viral RNA was present, and communicating that result to a smartphone.

The individual using the SLIDE platform simply projects saliva into a vial and onto a cartridge that is inserted into the device, and results are sent to a smartphone within 45 minutes.

The investigators reported that the SLIDE device achieved the limit of detection (LoD) of five copies/microliter of the saliva sample, which is comparable with the LoD (six copies/microliter) using FDA-approved quantitative real-time polymerase chain reaction (qRT-PCR) assays with the same heat-lysis saliva sample preparation method. With clinical samples, the SLIDE platform showed a good agreement with the results from the gold-standard RT-PCR method.

The investigators proposed that the SLIDE platform had great potential for performing self-administrated SARS-CoV-2 nucleic acid testing at home with noninvasive saliva samples.

The SLIDE device was described in the August 3, 2022, online edition of the journal ACS Sensors.

Related Links:
Pennsylvania State University 

 

Gold Supplier
SARS-CoV-2 Assay
INgezim COVID 19 SPIKE CROM
New
Dual-Channel Optical Coagulation Analyzer
Sensa2
New
Zika Virus IgM µ-Capture ELISA
https://www.ibl-international.com/en/zika-virus-igm-capture-elisa
New
Whole Blood Control
ESR-Chex Plus

Print article
IIR Middle East

Channels

Molecular Diagnostics

view channel
Image: Special blood test could determine if patient has early stage breast cancer and if the cancer is unlikely to return (Photo courtesy of USC)

Simple Blood Draw Could Revolutionize Early Breast Cancer Detection

Breast cancer is the most prevalent form of cancer in the world, affecting one in eight women over their lifetime. Since 1976 when the American Cancer Society endorsed mammography X-rays, the technique... Read more

Industry

view channel
Image: The global infectious disease IVD market is expected to hit USD 57 billion by 2030 (Photo courtesy of Pexels)

Global Infectious Disease IVD Market Dominated by Molecular Diagnostics Technology

The global infectious disease in vitro diagnostics (IVD) market stood at USD 113.7 billion in 2021 and is expected to grow at a CAGR of -7.41% from 2022 to 2030 to hit around USD 56.89 billion by 2030,... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.