We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics

Abbott Diagnostics

  Gold Abbott Diagnostics provides medical diagnostic instruments, tests, automation and informatics solutions, including cl... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Two Immunoassays Compared for Determination of Serum Vitamin D

By LabMedica International staff writers
Posted on 06 Jul 2021
Print article
Image: The IDS-iSYS Multi-Discipline Automated System Analyzer (Photo courtesy of Immunodiagnostic Systems)
Image: The IDS-iSYS Multi-Discipline Automated System Analyzer (Photo courtesy of Immunodiagnostic Systems)
Vitamin D is a fat-soluble steroid prohormone that is generated mainly in the skin by photochemical conversion of 7-dehydrocholesterol. 25-hydroxyvitamin D (25(OH)D) is its main storage form and is present in blood at very high concentration with respect to the active form 1,25-dihydroxyvitamin D.

The difficulties in the determination of 25(OH)D are related to its fat soluble nature, as well as its strong binding to the vitamin D binding protein (VDBP) and to serum albumin. On the other hand, although it would be appropriate for the different immunoassays to detect vitamin D2 and D3 in equimolar form, this is not usually fulfilled.

Clinical Biochemists at the Hospital Universitario Miguel Servet (Zaragoza, Spain) carried out a transversal descriptive observational study on a total of 184 individuals. The median age of the total of patients was 61 years old, with a minimum of 1 year and a maximum of 92. There were 31.5% men and 68.5% women. The samples came from both healthy patients and patients with various pathologies as bone, kidney, thyroid, cardiovascular, immune, neurological diseases and cancer.

The concentration of 25(OH)D was measured, as routine health analysis, by the automated IDS-iSYS 25VitD assay in an IDS-iSYS Multi-Discipline Automated System Analyzer (Vitro, Immunodiagnostic Systems Holdings PLC, Boldon Colliery, UK), which uses a chemiluminescence technology, which is fully automated. The same samples were processed by the Alinity i 25-OH Vitamin D assay in an Alinity i automated Analyzer (Abbott, Abbott Park, IL, USA), which is a one-step delayed-action immunoassay using chemiluminescent microparticles immunoassay (CMIA) technology.

The scientists reported that of the total patients, 52.7% received vitamin D supplements. The correlation coefficient of agreement for general group, supplementation group and group without supplementation was 0.92, 0.91 and 0.89, respectively. In all of them, a kappa index>0.75 was obtained. The mean value of the bias was higher for the supplemented group (−20.4 nmol/L) than for the group without treatment (−4.9 nmol/L). Graphically, a greater concordance between both assays was observed when patients receiving vitamin D supplements were excluded. The team observed lower concentrations of 25(OH)D in the IDS-iSYS 25 VitD assay when compared to the results obtained with the Alinity i 25-OH Vitamin D assay.

The authors concluded that the assays evaluated were not comparable to each other. Despite this, they show an excellent concordance in the evaluation of the vitamin D status. The study was published on June 23, 2021 in the journal Practical Laboratory Medicine.

Related Links:
Hospital Universitario Miguel Servet
Immunodiagnostic Systems Holdings PLC
Abbott


New
Gold Supplier
Desktop Urine Analyzer
MONOZYME TX2000
New
Nucleic Acid Purification System
CloNext 24
New
Silver Supplier
Immunoassay Reader
VEGA-200 Reader
New
3-Part Hematology Analyzer
H30 Pro

Print article

Channels

Molecular Diagnostics

view channel
Image: Model of the PD-1 (Programmed cell death protein 1) protein. Only a subset of recurrent glioblastomas respond to anti-PD-1 immunotherapy (Photo courtesy of Wikimedia Commons)

Biomarker Predicts Potential Benefit of Checkpoint Inhibitor Therapy for Brain Cancer Patients

A phosphorylated form of ERK (extracellular signal-regulated kinase) protein has been identified as a biomarker that may be used to predict which brain cancer patients might benefit from checkpoint inhibitor... Read more

Hematology

view channel
Image: The UniCel DxH 800 Coulter Cellular Analysis System (Photo courtesy of Beckman Coulter)

Monocyte Distribution Width Predicts Sepsis in Critically Ill Patients

Sepsis has been reported as a major cause of increased morbidity, length of stay and mortality among patients hospitalized in Intensive Care Units (ICUs) for any cause. The survival of patients developing... Read more

Immunology

view channel
Image: Procartaplex Immunoassays Kits are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously (Photo courtesy of Thermo Fisher Scientific)

Assay Developed for Patient-Specific Monitoring and Treatment for Ovarian Cancer

Tumors can influence peripheral immune macroenvironment, thereby creating opportunities for non-invasive serum/plasma immunobiomarkers for immunostratification and immunotherapy designing.... Read more

Microbiology

view channel
Image: Clinical metagenomics (CMg) using nanopore sequencing (Photo courtesy of Oxford Nanopore Technologies)

Same Day Test Identifies Secondary Infections in COVID-19 Patients

The intensive care unit (ICU) is a dynamic environment with frequent staff-patient contact for invasive monitoring, interventions and personal care that together introduce the risk of secondary or nosocomial... Read more

Pathology

view channel
Image: The Ventana BenchMark Ultra autostainer is for cancer diagnostics with automation and the test menu include IHC, ISH, and FITC tests (Photo courtesy of Ventana Medical System)

Specific Biomarker Investigated for Triple-Negative Breast Cancer Diagnosis

Triple-negative breast cancer (TNBC) is defined by the lack of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) expression and comprises a heterogeneous... Read more

Technology

view channel
Image: PKeye Workflow Monitor System (Photo courtesy of PerkinElmer, Inc.)

PerkinElmer’s New Cloud-Based Platform Enables Laboratory Personnel to Remotely Manage Its Instruments in Real-Time

PerkinElmer, Inc. (Waltham, MA; USA) has launched its PKeye Workflow Monitor, a cloud-based platform enabling laboratory personnel to remotely manage and monitor their PerkinElmer instruments and workflows... Read more

Industry

view channel
Illustration

Global HBA1c Laboratory Tests Market Driven by Rise in Diabetic Population

The global HBA1c laboratory tests market is projected to expand at a significant pace over the coming years, driven by an increase in the prevalence of diabetes, rise in prescription rate of HBA1c tests... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.