We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Abbott Diagnostics

Abbott Diagnostics provides medical diagnostic instruments, tests, automation and informatics solutions, including cl... read more Featured Products: More products

Download Mobile App




Effect of Lipoprotein(A) on the Diagnosis of Familial Hypercholesterolemia

By LabMedica International staff writers
Posted on 25 Nov 2019
Print article
Image: The Architect clinical chemistry autoanalyzer C16000 (Photo courtesy of Abbott Diagnostics).
Image: The Architect clinical chemistry autoanalyzer C16000 (Photo courtesy of Abbott Diagnostics).
The recent focus on familial hypercholesterolemia (FH) as a high-risk condition predisposing to premature coronary artery disease (CAD) has led to more widespread screening and diagnosis of the condition. Familial hypercholesterolemia is a genetic disorder.

FH is caused by a defect on chromosome 19. The defect makes the body unable to remove low density lipoprotein (LDL) cholesterol from the blood. Diagnostic tools for familial hypercholesterolemia (FH) rely on estimation of LDL cholesterol concentration. However, routine measurement or calculation of LDL cholesterol concentration using the Friedewald equation contains a cholesterol contribution from lipoprotein(a) [Lp(a)].

Medical Scientists from the University of Western Australia (Perth, Australia) undertook a cross-sectional study of adult index patients aged >18 years with or without a recognized mutation causative of FH. Data on Lp(a) concentrations were available in 907 patients suspected of FH. The Dutch Lipid Clinic Network (DLCN) and Simon Broome (SB) diagnostic criteria were estimated before and after adjusting LDL cholesterol concentration for the cholesterol content (30%) of Lp(a).

All biochemical measurements were performed with routine assays in an accredited laboratory. LDL cholesterol was estimated by the Friedewald equation; with triglyceride >400 mg/dL (4.5 mmol/L) (n = 4), LDL cholesterol was measured by direct assay. LDL cholesterol was adjusted for statin therapy in individuals receiving therapy to establish the phenotypic diagnosis of FH. Lp(a) was measured by an automated latex enhanced immunoassay, the Quantia Lp(a) assay, (Abbott Laboratories, Abbott Park, IL, USA). Briefly, the Quantia Lp(a) is a turbidimetric immunoassay using monoclonal antibody for the estimation of Lp(a) in human serum or plasma in an Abbott Diagnostics Architect autoanalyzer C16000 and is based on the principle of an agglutination reaction.

The investigators reported that 74 patients defined by DLCN criteria (8.2%) and 207 patients defined by SB criteria (22.8%) were reclassified to “unlikely” FH after adjusting LDL cholesterol for Lp(a) cholesterol. The proportion of FH patients defined by DLCN (probable/definite) and SB (possible/definite) criteria decreased significantly in patients with increased Lp(a) (>0.5 g/L; n = 330) after Lp(a) cholesterol adjustment. The overall reclassification rate was significantly higher in patients with Lp(a) concentration >1.0 g/L. The AUROC curve for LDL cholesterol concentration ≥191 mg/dL (≥5.0 mmol/L), DLCN criteria, and SB criteria in predicting an FH mutation increased significantly after adjustment. There was no significant difference in AUROC curve before and after Lp(a) cholesterol adjustment at an LDL cholesterol concentration ≥251 mg/dL (≥6.5 mmol/L).

The authors concluded that adjusting LDL cholesterol concentration for Lp(a) cholesterol improves the diagnostic accuracy of DLCN and SB criteria, especially with Lp(a) >1.0 g/L and LDL cholesterol <251 mg/dL (<6.5 mmol/L). Lp(a) should be measured in all patients suspected of having FH. The study was published in the October, 2019 issue of the journal Clinical Chemistry.

Related Links:
University of Western Australia
Abbott Laboratories


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
One Step HbA1c Measuring System
GREENCARE A1c
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
Gold Member
Real-time PCR System
GentierX3 Series

Print article

Channels

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.