We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

THE BINDING SITE

Engages in the research, development, manufacture, and distribution of immunodiagnostic assays, as wel as specialized... read more Featured Products: More products

Download Mobile App





IL-1RA Antibodies in Myocarditis After SARS-CoV-2 Vaccination

By LabMedica International staff writers
Posted on 26 Sep 2022
Print article
Image: IL-1RA antibody levels measured as a biomarker in young males with myocarditis after SARS-CoV-2 vaccination (Photo courtesy of Patrice Wendling)
Image: IL-1RA antibody levels measured as a biomarker in young males with myocarditis after SARS-CoV-2 vaccination (Photo courtesy of Patrice Wendling)

Myocarditis associated with messenger RNA (mRNA) vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) predominantly affects male adolescents and young male adults (14 to <30 years of age) and typically occurs after receipt of the second vaccine dose.

In adults with critical coronavirus disease 2019 (Covid-19) and in cases of multisystem inflammatory syndrome in children (MIS-C), it has recently been discovered that neutralizing autoantibodies targeting the endogenous interleukin-1 receptor antagonist (IL-1RA), which inhibits interleukin-1 signaling and inflammation.

A large team of Medical Scientists led by those at Saarland University (Homburg, Germany) evaluated the prevalence of antibodies neutralizing IL-1RA and progranulin, which inhibits tumor necrosis factor signaling, in 69 patients (14 to 79 years of age) who had clinically suspected myocarditis after SARS-CoV-2 vaccination. A total of 61 patients underwent endomyocardial biopsy. Myocarditis was confirmed by biopsy in 40 of 61 patients.

For immunohistological detection of cardiac immune cells, a monoclonal rabbit-anti-CD3 antibody (Novocastra Laboratories, Newcastle upon Tyne, UK), a monoclonal mouse anti-human CD68 antibody and a monoclonal mouse anti-human HLA-DR alpha-chain antibody (DAKO, Hamburg, Germany) were used. Immunohistochemical analysis was performed on an automated immunostainer following the manufacturer’s protocol (Benchmark; Ventana Medical Systems, Tucson, AZ, USA) and using the Ventana ultraView detection system and diaminobenzidine as substrate. ELISA for antibodies was performed including assays for IgG1, IgG2, IgG3 and IgG4 (Binding Site Group, Birmingham, UK) and IL-1-Ra plasma levels were determined with a commercially available ELISA kit (Invitrogen/ThermoFisher, Carlsbad, CA; USA). Isoelectric focusing (IEF) and Western blotting (including native Western blotting with non-reducing sample pretreatment and gradient gels without SDS) was also performed.

The investigators reported that among patients with histologically confirmed myocarditis, anti–IL-1RA antibodies were found in 9/12 patients (75%) younger than 21 years of age, as compared with 3/28 patients (11%) 21 years of age or older. Anti–IL-1RA antibodies were not detectable in the 21 patients in whom biopsy ruled out the diagnosis of myocarditis. IL-1RA antibody–positive patients with biopsy-confirmed myocarditis had an early onset of symptoms, which occurred mostly after receipt of the second vaccine dose, and a milder course of myocarditis than patients with biopsy-confirmed myocarditis but without anti–IL-1RA autoantibodies.

At the time of acute myocarditis, the mean (±SD) free IL-1RA plasma level in 15 patients who were seropositive for anti–IL-1RA antibodies was 236 ± 82 pg/mL, whereas the level was 1,736 ± 312 pg/mL in 33 patients without anti–IL-1RA antibodies and 1,599 ± 277 pg/mL in 21 patients in whom histologic testing ruled out the diagnosis of myocarditis. IL-1RA plasma levels correlated with markers of cardiac damage (troponin T, creatine kinase, creatine kinase MB, or pro–B-type natriuretic peptide), cardiac-tissue infiltration of CD3+ T cells and CD68+ macrophages, and systemic inflammation (C-reactive protein).

The authors concluded that in their study of SARS-CoV-2 vaccination–associated myocarditis and anti–IL-1RA antibodies should be interpreted within the context that the transiency of hyperphosphorylation and patients’ HLA haplotypes were not known. Neutralizing antibodies against IL-1RA and a hyperphosphorylated IL-1RA isoform were observed in young male patients with biopsy-confirmed myocarditis after the receipt of SARS-CoV-2 mRNA vaccine. These antibodies impaired IL-1RA bioactivity in vitro, were associated with low circulating levels of IL-1RA, and were found in patients with biomarker evidence of cardiac damage and inflammation. The study was published on September 21, 2022 in the journal the New England Journal of Medicine.

Related Links:
Saarland University
Novocastra Laboratories
DAKO
Ventana Medical Systems
Binding Site Group
Invitrogen/ThermoFisher

New
Platinum Member
Flu SARS-CoV-2 Combo Test
OSOM® Flu SARS-CoV-2 Combo Test
Magnetic Bead Separation Modules
MAG and HEATMAG
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
Gold Member
SARS-CoV-2 RT-PCR Assay
Reliance SARS-CoV-2 RT-PCR Assay Kit

Print article

Channels

Clinical Chemistry

view channel
Image: The new ADLM guidance will help healthcare professionals navigate respiratory virus testing in a post-COVID world (Photo courtesy of 123RF)

New ADLM Guidance Provides Expert Recommendations on Clinical Testing For Respiratory Viral Infections

Respiratory tract infections, predominantly caused by viral pathogens, are a common reason for healthcare visits. Accurate and swift diagnosis of these infections is essential for optimal patient management.... Read more

Molecular Diagnostics

view channel
Image: The HelioLiver Dx test has met the coprimary and secondary study endpoints in the CLiMB trial (Photo courtesy of Helio Genomics)

Blood-Based Test Outperforms Ultrasound in Early Liver Cancer Detection

Patients with liver cirrhosis and chronic hepatitis B are at a higher risk for developing hepatocellular carcinoma (HCC), the most prevalent type of liver cancer. The American Association for the Study... Read more

Hematology

view channel
Image: The CAPILLARYS 3 DBS devices have received U.S. FDA 510(k) clearance (Photo courtesy of Sebia)

Next Generation Instrument Screens for Hemoglobin Disorders in Newborns

Hemoglobinopathies, the most widespread inherited conditions globally, affect about 7% of the population as carriers, with 2.7% of newborns being born with these conditions. The spectrum of clinical manifestations... Read more

Microbiology

view channel
Image: The POC PCR test shortens time for STI test results (Photo courtesy of Visby Medical)

POC STI Test Shortens Time from ED Arrival to Test Results

In a 2024 sexually transmitted infections (STIs) surveillance report by the World Health Organization (WHO), over 2.5 million cases were recorded, alongside a rise in the inappropriate use of antibiotics... Read more

Industry

view channel
Image: For 46 years, Roche and Hitachi have collaborated to deliver innovative diagnostic solutions (Photo courtesy of Roche)

Roche and Hitachi High-Tech Extend 46-Year Partnership for Breakthroughs in Diagnostic Testing

Roche (Basel, Switzerland) and Hitachi High-Tech (Tokyo, Japan) have renewed their collaboration agreement, committing to a further 10 years of partnership. This extension brings together their long-standing... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.