Stem Cells Used to Generate Functional Arterial Endothelial Cells

They then manipulated these pathways and generated arterial endothelial cells that demonstrated unprecedented arterial-specific functions as well as improved survival of myocardial infarction.

Single-cell RNA sequencing identified the signaling pathways - involving 40 genes of optimal relevance - critical for arterial endothelial cell differentiation. CRISPR-Cas9 gene editing technology allowed the creation of reporter cell lines to monitor arterial differentiation in real time.

The investigators reported in the July 10, 2017, online edition of the journal Proceedings of the [U.S.] National Academy of Sciences that by using these protocols they were able to produce endothelial cells from multiple human embryonic and induced pluripotent stem cell lines with gene expression profiles, oxygen consumption rates, nitric oxide production levels, shear stress responses, and TNFalpha-induced leukocyte adhesion rates characteristic of arterial endothelial cells.

"Our ultimate goal is to apply this improved cell derivation process to the formation of functional arteries that can be used in cardiovascular surgery," said senior author Dr. James Thomson, professor of cell and regenerative biology at the Morgridge Institute for Research. "This work provides valuable proof that we can eventually get a reliable source for functional arterial endothelial cells and make arteries that perform and behave like the real thing."

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Morgridge Institute for Research