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适合中型实验室的细菌化验

By LabMedica International staff writers
Posted on 07 Aug 2018
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图片:iC-GPC革兰氏阳性测定是一种定性的多重检验,可直接检测阳性血液培养物中的五种最常见的革兰氏阳性菌,它们引起细菌性血流感染(图片蒙iCubate惠赐)。
图片:iC-GPC革兰氏阳性测定是一种定性的多重检验,可直接检测阳性血液培养物中的五种最常见的革兰氏阳性菌,它们引起细菌性血流感染(图片蒙iCubate惠赐)。

细菌性脓毒症及相关并发症是病症的一大原因,也是美国的第十大死因。早期诊断并实施恰当的抗生素疗法对于病人的良好预后至关重要。

 

传统的培养法和抗生素敏感性试验方法是依赖于时间的过程,往往需要二至四天才能得到最终检验结果。最近开发的一款测定产品能准确可靠地检测五种最常见的革兰氏阳性菌,它们引起细菌性血流感染。

 

美国纽约州锡拉丘兹市的中纽约实验室联盟(www.laboratoryalliance.com)率领来自不同单位的一组医疗化验科学家,从20151月至201610月收集并检验了1,134名患者的血液培养临床样本。评估了三种不同的血液自动培养系统:美国北卡罗莱纳州杜勒姆市生物梅里埃股份有限公司(www.biomerieux-usa.com)BacT/ALERT;美国马里兰州科基斯维尔市碧迪公司(www.bd.com)BACTEC;美国马萨诸塞州沃尔瑟姆市赛默飞世尔科技公司(www.thermofisher.com)VersaTREK。科学家们比较了上述产品与美国阿拉巴马州亨茨维尔市iCubate股份有限公司(wwwHYPERLINK "http://www.icubate.com/".HYPERLINK "http://www.icubate.com/"icubate.com)iC-GPC测定。

 

iC-GPC革兰氏阳性测定是一种定性的多重检验,可直接检测阳性血液培养物中引起细菌性血流感染的五种最常见的革兰氏阳性菌:金黄色葡萄球菌、表皮葡萄球菌、肺炎链球菌、粪肠球菌和屎肠球菌。通过多中心临床实验检验病人含革兰氏阳性球菌的血液培养物样本,该测定产品还检测到决定mecAvanAvanB耐药性的因素。

 

iC-GPC测定的总体百分比与美国德克萨斯州奥斯汀市Luminex公司(www.luminexcorp.com)BC-GP测定一致,为95.5%。通过聚合酶链反应(PCR)和双向测序方法进一步分析不一致的样本。结果说明iC-GPC测定再加上iCubate系统能准确可靠地检测血流感染中五种最常见的革兰氏阳性菌及其耐药标志物。

 

该研究的论文首先发表于2018613日的《临床微生物学杂志》(Journal of Clinical Microbiology)。作者总结说,iC-GPC测定提供了一种便宜得多的替代方案,尤其是对于中小规模医疗机构,实现血流感染常见革兰氏阳性菌及其耐药标志物的分子检测。

 

论文的领衔作者、微生物系主任Paul A. Granato博士说:脓毒血症是引起血流感染的原因之一,传统的诊断或检测方法需要二至五天才能鉴定细菌,确定哪类抗生素最适合治疗患者。

Related Links:

中纽约实验室联盟>>> www.laboratoryalliance.com

生物梅里埃>>> www.biomerieux-usa.com

碧迪公司>>> www.bd.com

赛默飞世尔科技公司>>> www.thermofisher.com

iCubate股份有限公司>>> wwwHYPERLINK "http://www.icubate.com/".HYPERLINK "http://www.icubate.com/"icubate.com

Luminex公司 >>> www.luminexcorp.com



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