We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




RT-LAMP Used to Genotype and Diagnose Chikungunya

By LabMedica International staff writers
Posted on 27 Jun 2019
Print article
Image: Blue-stained dots are Chikungunya virus particles peppering the surface of an infected cell (Photo courtesy of Institute Pasteur/Thérèse Couderc).
Image: Blue-stained dots are Chikungunya virus particles peppering the surface of an infected cell (Photo courtesy of Institute Pasteur/Thérèse Couderc).
Chikungunya virus has re-emerged as an important pathogen causing several outbreaks in the world. As the clinical symptoms of chikungunya are similar to other mosquito-borne febrile diseases, the definitive diagnosis of the disease is based on the detection of viral genome from the patient blood.

Loop-mediated isothermal amplification (LAMP) is a method that rapidly amplifies nucleic acids under isothermal condition. The method can be applied to detect genomes of RNA viruses if reverse transcriptase is included in the reaction (reverse transcription LAMP; RT-LAMP). Using the LAMP and RT-LAMP methods, amplification reaction of nucleic acids can be performed under isothermal conditions without expensive or sophisticated equipment.

An international team of scientists working with Hokkaido University (Sapporo, Japan) detected and sequenced the chikungunya virus (CHIKV) genome by performing a combination of a modified reverse transcription loop-mediated isothermal amplification (RT-LAMP) method and a MinION sequencer. They developed the protocol for drying all the reagents for the RT-LAMP in a single reaction tube.

The team found that using this system, the CHIKV genome was effectively amplified under isothermal conditions, and used as a template for MinION sequencing with a laptop computer. The assay was also validated using clinical samples. Serum samples were obtained from clinically suspected patients collected in 2016 and 2018, and which had been stored at the Oswaldo Cruz Institute. The obtained sequence data confirmed the CHIKV outbreaks and identified the genotype.

The authors concluded that a one-step, easy gene amplification assay for CHIKV genome detection was successfully developed. The assay was evaluated with RNA and serum samples from 16 CHIKV serology positive patients during recent chikungunya outbreaks in Rio de Janeiro, Brazil. In combination with MinION sequencing technology, they also identified the CHIKV genotypes with a laptop computer. The developed CHIK-CZC-LAMP diagnostics and MinION sequencing workflow will certainly contribute to future outbreak analysis in resource-limited settings. The study was published on June 3, 2019, in the journal PLOS Neglected Tropical Diseases.

Related Links:
Hokkaido University

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
HLX
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
New
Gold Member
Fully Automated Cell Density/Viability Analyzer
BioProfile FAST CDV

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.