We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us

Download Mobile App

Novel Microscopy Technique Comes Closer to Use in Clinical Diagnostics

By LabMedica International staff writers
Posted on 14 Mar 2023
Print article
Image: New research is set to bring Brillouin microscopy closer to widespread use in diagnostic medicine (Photo courtesy of Wayne State University)
Image: New research is set to bring Brillouin microscopy closer to widespread use in diagnostic medicine (Photo courtesy of Wayne State University)

Diagnostic imaging plays a crucial role in aiding physicians and researchers in understanding internal body structures, thus improving clinical analysis and medical intervention. Scientists continually explore new avenues to utilize imaging technologies to gain insight into human health. A pioneering imaging method called Brillouin microscopy enables the mapping of cell and tissue stiffness, often linked to early symptoms of ailments such as cancer and Alzheimer's. This method is distinct from conventional imaging modalities such as confocal fluorescence microscopy, as it allows label-free and non-contact acquisition of key mechanical information like viscosity and stiffness of biological specimens. Now, researchers are striving to refine Brillouin microscopy, which can answer many important questions in biophysics and mechanobiology.

Brillouin microscopy, an optical imaging method rooted in Brillouin light scattering (BLS), was first introduced by French physicist Léon Brillouin in 1922. When light interacts with a substance, thermal fluctuations or molecular vibrations within the material cause the light to scatter resulting in BLS. These vibrations can be influenced by various factors such as compression, water content, heat, or material stiffness. Among these factors, stiffness is incredibly valuable for the diagnostic application of Brillouin microscopy. Changes in cell stiffness, often linked to the progression of ailments like cancer metastasis, are challenging to measure since cells are microscopic and situated in very delicate tissues.

In conventional approaches, prepared cells are measured on a petri dish or other rigid substrates. However, Brillouin microscopy relies solely on a laser beam to investigate the mechanical properties, enabling measurement when cells are in their physiological conditions. As no physical interaction is required, Brillouin technology is less invasive and more convenient. The technology is important for understanding embryonic tissue development, particularly to gain a better understanding of birth-related diseases and disorders.

Researchers at Wayne State University (Detroit, MI, USA) examined the use of dual line-scanning Brillouin microscopy (dLSBM) to overcome two significant limitations - acquisition speed and irradiation doses - that hinder its widespread usage in biomedicine. The application of dLSBM yielded 50 to 100 times faster speeds than its counterpart, with a reduction of 80 times light irradiation levels for 2D and 3D mechanical mapping.

“With this innovation, we can acquire one mechanical image of cell clusters in a few minutes,” said Jitao Zhang, assistant professor of biomedical engineering (BME) at Wayne State University. “This improved acquisition speed is important because it allows us to investigate details of cell behaviors in almost real time.”

“Due to the 3D structure of an embryo, traditional contact-based techniques encounter big challenges for in vivo measurement,” added Zhang. “Since Brillouin microscopy works in a non-contact manner, it sometimes becomes the only available choice.”

Related Links:
Wayne State University

Platinum Member
Flu SARS-CoV-2 Combo Test
OSOM® Flu SARS-CoV-2 Combo Test
Magnetic Bead Separation Modules
POCT Fluorescent Immunoassay Analyzer
Gold Member
Hemoglobin Testing System

Print article


Clinical Chemistry

view channel
Image: The new ADLM guidance will help healthcare professionals navigate respiratory virus testing in a post-COVID world (Photo courtesy of 123RF)

New ADLM Guidance Provides Expert Recommendations on Clinical Testing For Respiratory Viral Infections

Respiratory tract infections, predominantly caused by viral pathogens, are a common reason for healthcare visits. Accurate and swift diagnosis of these infections is essential for optimal patient management.... Read more

Molecular Diagnostics

view channel
Image: The HelioLiver Dx test has met the coprimary and secondary study endpoints in the CLiMB trial (Photo courtesy of Helio Genomics)

Blood-Based Test Outperforms Ultrasound in Early Liver Cancer Detection

Patients with liver cirrhosis and chronic hepatitis B are at a higher risk for developing hepatocellular carcinoma (HCC), the most prevalent type of liver cancer. The American Association for the Study... Read more


view channel
Image: The CAPILLARYS 3 DBS devices have received U.S. FDA 510(k) clearance (Photo courtesy of Sebia)

Next Generation Instrument Screens for Hemoglobin Disorders in Newborns

Hemoglobinopathies, the most widespread inherited conditions globally, affect about 7% of the population as carriers, with 2.7% of newborns being born with these conditions. The spectrum of clinical manifestations... Read more


view channel
Image: The POC PCR test shortens time for STI test results (Photo courtesy of Visby Medical)

POC STI Test Shortens Time from ED Arrival to Test Results

In a 2024 sexually transmitted infections (STIs) surveillance report by the World Health Organization (WHO), over 2.5 million cases were recorded, alongside a rise in the inappropriate use of antibiotics... Read more


view channel
Image: For 46 years, Roche and Hitachi have collaborated to deliver innovative diagnostic solutions (Photo courtesy of Roche)

Roche and Hitachi High-Tech Extend 46-Year Partnership for Breakthroughs in Diagnostic Testing

Roche (Basel, Switzerland) and Hitachi High-Tech (Tokyo, Japan) have renewed their collaboration agreement, committing to a further 10 years of partnership. This extension brings together their long-standing... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.