We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Latex Agglutination Test Identifies Bacterial Groups

By LabMedica International staff writers
Posted on 06 Dec 2011
The latex agglutination kit offers a faster streptococci-grouping solution than traditional methods for all clinically significant groups. More...


The kit is designed to improve workflow efficiencies since the nitrous acid extraction instantaneously extracts the cell antigens and the test does not require rehydration or incubation.

Beta-hemolytic streptococci may be identified rapidly and accurately according to the presence of specific cell wall antigens in a process called Lancefield Grouping. This process is most conveniently carried out using latex agglutination. To prepare the antigens for a latex agglutination reaction, an enzyme-mediated or nitrous acid extraction method may be used. Commercially available nitrous acid extraction kits are not traditionally capable of detecting group D antigen.

The PathoDxtra Strep Grouping Kit a latex agglutination kit from Thermo Fisher Scientific (Basingstoke, UK), detects groups A, B, C, D, F, and G antigen using a nitrous acid extraction method. The company report that a test panel of 131 beta-hemolytic streptococci have been assayed, including: Lancefield 21 group A, 27 B, 20 C , 23 D, 20 F, and 20 G. Thirty non-Lancefield group isolates were also tested, including: Streptococcus pneumoniae, S. viridans, Staphylococcus aureus, and Listeria monocytogenes. The kit was compared with other commercial kits.

All kits showed 100% accuracy in identifying all Lancefield group A and group C isolates. For the remaining isolates, PathoDxtra Strep Grouping Kit performed better than or comparable to all other products examined. The overall performance of kits that included a group D latex showed that PathoDxtra Strep Grouping Kit correctly identified 150/161 (93.2%) of the isolates tested. PathoDxtra Strep Grouping Kit agglutination reactions were consistently strong and exhibited a fast agglutination reaction for all Lancefield groups, providing results within 20 seconds.

Julie Elston, global product manager, immunodiagnostics, at Thermo Fisher said, "Acid extraction kits offered speed but did not detect group D strep reliably, while enzyme kits detected all groups but required rehydration and incubation steps, adding significant time to testing procedures. With PathoDxtra there is no compromise, this kits gives instantaneous extraction and detects all clinically significant streps reliably, including group D. It really couldn’t be simpler.”

Related Links:
Thermo Fisher Scientific



Gold Member
Quantitative POC Immunoassay Analyzer
EASY READER+
3-Part Differential Hematology Analyzer
Swelab Alfa Plus Sampler
New
Capillary Blood Collection Tube
IMPROMINI M3
New
Sperm Quality Analyis Kit
QwikCheck Beads Precision and Linearity Kit
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








DIASOURCE (A Biovendor Company)

Channels

Molecular Diagnostics

view channel
Image: A diagnostic test can distinguish patients with head and neck squamous cell carcinoma who can be cured with surgery alone (Photo courtesy of University of Turku)

Novel Diagnostic Tool to Revolutionize Treatment Guidance of Head and Neck Cancer

Head and neck squamous cell carcinoma (HNSCC) is a solid tumor type commonly treated with surgery. However, there has been no clinically available method to determine which patients can be cured with surgery... Read more

Hematology

view channel
Image: The microfluidic device for passive separation of platelet-rich plasma from whole blood (Photo courtesy of University of the Basque Country)

Portable and Disposable Device Obtains Platelet-Rich Plasma Without Complex Equipment

Platelet-rich plasma (PRP) plays a crucial role in regenerative medicine due to its ability to accelerate healing and repair tissue. However, obtaining PRP traditionally requires expensive centrifugation... Read more

Immunology

view channel
Image: The 3D paper-based analytical device has shown high clinical accuracy for adult-onset immunodeficiency (Photo courtesy of National Taiwan University)

Paper-Based Device Accurately Detects Immune Defects in 10 Minutes

Patients with hidden immune defects are especially vulnerable to severe and persistent infections, often due to autoantibodies that block interferon-gamma (IFN-γ), a key molecule in immune defense.... Read more

Technology

view channel
Image: The Check4 gene-detection platform (Photo courtesy of IdentifySensors)

Electronic Biosensors Used to Detect Pathogens Can Rapidly Detect Cancer Cells

A major challenge in healthcare is the early and affordable detection of serious diseases such as cancer. Early diagnosis remains difficult due to the complexity of identifying specific genetic markers... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.