Infrared Laser Detects Malaria Parasites in Blood

Most common in tropical and subtropical regions, there are 350 to 500 million new cases--and one to three million fatalities--reported annually. Most of the fatalities are concentrated in sub-Saharan Africa, where the resources and trained personnel currently required to accurately diagnose the disease are spread the thinnest.


Current detection techniques require trained technicians to stain slides, look for the parasite's DNA signature under the microscope, and then manually count all the visible infected cells, a laborious process dependent on the skill and availability of trained analysts. By contrast, the proposed new technique relies on a known optical effect called third harmonic generation (THG), which causes hemozoin--a crystalline substance secreted by the parasite--to glow blue when irradiated by an infrared laser.

Dr. Paul Wiseman of the departments of physics and chemistry at McGill University said, "People who are familiar with music know about acoustic harmonics. You have a fundamental sound frequency and then multiples of that frequency. Non-linear optical effects are similar: if you shine an intense laser beam of a specific frequency on certain types of materials, you generate multiples of the frequency. Hemozoin has a huge, non-linear optical response for the third harmonic, which causes the blue glow.

A team of scientists led by Dr. Wiseman of the departments of physics and chemistry at McGill University (Montreal, Quebec) developed the radically new technique, which was described in the December 2007 issue of Biophysical Journal. The scientists say the new technique holds the promise of simpler, faster, and far less labor-intensive detection of the malaria parasite in blood samples.

Dr. Wiseman and his colleagues now plan to adapt well-established existing technologies like fiber-optic communications lasers and fluorescent cell sorters to quickly move the technique out of the laboratory and into the field.

We [are] imagining a self-contained unit that could be used in clinics in endemic countries, said Dr. Wiseman. The operator could inject the cell sample directly into the device, and then it would come up with a count of the total number of existing infected cells without manual intervention.


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Departments of physics and chemistry at McGill University