We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us


Qiagen is a provider of sample and assay technologies for molecular diagnostics and applied testing, including comple... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
22 May 2021 - 26 May 2021
Virtual Venue
25 May 2021 - 28 May 2021

IFN‐γ Release Assay Helps Diagnose Hemophagocytic Lymphohistiocytosis

By LabMedica International staff writers
Posted on 14 Aug 2020
Print article
The QuantiFERON‐TB Gold Plus IFN‐γ release assay used to help diagnose hemophagocytic lymphohistiocytosis (Photo courtesy of Qiagen).
The QuantiFERON‐TB Gold Plus IFN‐γ release assay used to help diagnose hemophagocytic lymphohistiocytosis (Photo courtesy of Qiagen).
Hemophagocytic lymphohistiocytosis (HLH), either primary (pHLH, or Familiar, FHL) or secondary is a life‐threatening hyper‐inflammatory syndrome, characterized by massive and uncontrolled activation of macrophages and T cells, causing fever, cytopenia and liver dysfunction with coagulopathy.

The rarity of the HLH disease, along with its pleomorphic clinical presentation that mimics a number of different conditions (from severe infections to malignancies), makes the diagnosis challenging. Hemophagocytosis on bone morrow biopsy is the only histomorphological criterion, but, in itself, it is neither specific nor sensitive.

Hematologists at the Bambino Gesù Children's Hospital (Rome, Italy) diagnosed and treated 13 children with pHLH at the hospital from August 2014 to April 2020. During the diagnostic evaluation, all patients were tested for tuberculosis (TB) exposure through QuantiFERON‐TB Gold (QFT‐G, Qiagen, Hilden, Germany). The QuantiFERON test is an IFN‐γ release assay (IGRA), which measures IFN‐γ release in response to tuberculosis (TB)‐specific antigens. After centrifugation, plasma was harvested and enzyme‐linked immunosorbent assay (ELISA) tests were run.

The scientists reported that results obtained from the HLH group (all tests were reported as ‘Indeterminate’ because of high levels of IFN‐γ in the NIL Tube) were compared with three other groups of pediatric patients (matched for gender) with an available QFT‐G. These groups were: 13 children hospitalized for acute leukemia (AL) at time of diagnosis, 13 because of sepsis and 13 healthy controls (HC). The median (range) IFN‐γ levels at diagnosis/before starting treatment in the HLH group was 2.49 IU/mL (0.12–15.11) , significantly higher than those found in the AL, sepsis and HC groups, in which the median (range) value was 0.04 IU/mL (0.02–0.11) 0.07 (IU/mL 0.03–0.15) and 0.036 IU/mL (0.015–0.077) , respectively. A cut‐off value of 0.12 IU/mL had a sensitivity of 100% and a specificity of 97.3%.

The authors concluded that QFT‐G Plus, a diffuse and standardized blood test, in association with HLH‐04 criteria could support clinicians in determining a diagnosis of pHLH with high sensitivity and specificity. Although other interferonopathies, genetic or acquired, such as systemic lupus erythematosus and juvenile dermatomyositis, could impair the specificity of the analysis, this simple diagnostic test could really support pediatricians in peripheral hospitals. The study was published on July 26, 2020 in the British Journal of Haematology.

Related Links:

Bambino Gesù Children's Hospital
Gold Supplier
COVID-19 Antigen Test
Rapid COVID-19 Antigen Test
Flow Cytometer
Amnis CellStream
SARS-CoV-2 Antigen Test
NG Test SARS-CoV-2 Ag
SARS-CoV-2 S1/S2 IgG Assay

Print article
Mayo Medical Laboratories
BIOHIT  Healthcare OY


Molecular Diagnostics

view channel
Image: Photograph of the novel capsule-based smell test (Photo courtesy of Queen Mary University of London)

A Novel Capsule-Based Smell Test for Diagnosis of Neurological and Respiratory Diseases

To aid in diagnosing diseases where loss of the sense of smell is a symptom, such as in chronic neurological conditions like Parkinson's and Alzheimer's diseases and in acute respiratory infections such... Read more


view channel
Image: The Hamamatsu Photonics Nanozoomer 1 Digital Slide Scanner (Photo courtesy of University of Adelaide)

Genetic Background and Clinicopathologic Features Established for Adult-Onset Nephronophthisis

Nephronophthisis (NPH) is a genetic disorder of the kidneys which mainly affects children. It is classified as a medullary cystic kidney disease. The disorder is inherited in an autosomal recessive fashion... Read more


view channel
Image: DxA 5000 Fit (Photo courtesy of Beckman Coulter)

Beckman Coulter Becomes First Diagnostics Company to Offer Workflow Automation Specifically for Mid-Volume Laboratories

Beckman Coulter (Brea, CA, USA) has announced the global launch of the DxA 5000 Fit, a workflow-automation solution designed to fit into medium-sized labs that run fewer than 5,000 tests a day.... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.