Prolonged Live-Cell Incubation Visualized Using Fluorescence Microscope

Because the system is based on a true incubator, physical stress on the cultures is minimized and cells remain uncontaminated in a healthy environment throughout the observation process.

Imaging takes place within the incubator, and eight vessels at a time are handled on the motorized stage, allowing maximum compliance with a wide variety of experimental protocols. A liquid handling option allows the scientist to add agonists or stimulants to the cell cultures with a minimum disturbance of the environment.

The system can handle observation of multiple locations in the eight dishes simultaneously, for up to a week at a time. This enables the study of morphology and protein expression in sensitive cell types. The closed-box system incorporates an incubator, fully motorized inverted microscope, high-performance non-drift optics, cooled charged-coupled device (CCD) camera, and computer software. It offers excellent flexibility and optical performance, and is simple to use.

The new system, called the LCV110 Incubator Fluorescence Microscope, was developed by Olympus America (Center Valley, PA, USA). The microscope was designed for use with a 40x, 0.95 numerical aperture (NA) dry objective, and it is suitable for time-lapse imaging of live cells.

Olympus serves healthcare and commercial laboratory markets with integrated product solutions and financial, educational, and consulting services. The company develops technologies with new product design and functionality for the consumer and professional photography markets, and is a leader in gastrointestinal endoscopy, and in clinical and educational microscopes.

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