We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




New DNA Testing Method Offers Faster and More Accurate Pathogen Identification

By LabMedica International staff writers
Posted on 05 Jun 2024
Print article
Image: The new method offers faster, more accurate pathogen identification, even in complex DNA sequences (Photo courtesy of 123RF)
Image: The new method offers faster, more accurate pathogen identification, even in complex DNA sequences (Photo courtesy of 123RF)

The Polymerase Chain Reaction (PCR) is widely regarded as the definitive method for target DNA sequence amplification, testing, and analysis. In this traditional PCR process, the DNA sample undergoes heating to separate into single strands, which then serve as templates for synthesizing new DNA strands through enzymatic action. Despite its effectiveness, this method can be cumbersome, slow, and costly. Now, researchers have introduced an alternative DNA testing method that could potentially replace traditional PCR, paving the way for broader applications in medical diagnostics.

Developed at Case Western Reserve University (Cleveland, OH, USA), this new technique or reaction is named AMPLON (Amplifying DNA with Multiarm Priming and Looping Optimization of Nucleic Acid). It enables comparison of DNA from diseased cells with that from healthy ones, enhancing understanding of disease progression and treatment approaches. AMPLON uses multiple extensions along the DNA strand, significantly enhancing the speed and accuracy of DNA synthesis at a constant temperature. This simplified method avoids the thermal stress typically imposed on materials by traditional PCR's fluctuating temperatures.

Furthermore, AMPLON offers a more organized and practical amplification method, particularly beneficial in environments where maintaining precise temperature control is difficult. The innovative design of its multi-armed DNA primers turns the limitations associated with enzymatic reactions into advantages, increasing the efficiency of the amplification process and ensuring consistent results. This new technique holds promise for transforming molecular analysis and clinical diagnostics across various fields, including infectious disease diagnostics, personalized medicine, and environmental monitoring. 

“We’ve developed a new method of DNA amplification that does not require bulky lab-bound equipment but can be conducted in one step and in diverse settings. More significantly, our approach does not weaken enzymes like the PCR method,” said Mohamed S. Draz, an assistant professor at Case Western Reserve's School of Medicine and the principal investigator of the study, which was recently published in the journal Advanced Materials. “We’ve been able to enhance amplification and reduce amplification time by 50%. Our approach has the potential to dramatically change the way nucleic acid amplification is performed, providing instead a portable, reliable and cost-effective solution for applications, ranging from point-of-care diagnostics to field-based research.” 

Related Links:
Case Western Reserve University


Print article

Channels

Clinical Chemistry

view channel
Image: The new ADLM guidance will help healthcare professionals navigate respiratory virus testing in a post-COVID world (Photo courtesy of 123RF)

New ADLM Guidance Provides Expert Recommendations on Clinical Testing For Respiratory Viral Infections

Respiratory tract infections, predominantly caused by viral pathogens, are a common reason for healthcare visits. Accurate and swift diagnosis of these infections is essential for optimal patient management.... Read more

Hematology

view channel
Image: The CAPILLARYS 3 DBS devices have received U.S. FDA 510(k) clearance (Photo courtesy of Sebia)

Next Generation Instrument Screens for Hemoglobin Disorders in Newborns

Hemoglobinopathies, the most widespread inherited conditions globally, affect about 7% of the population as carriers, with 2.7% of newborns being born with these conditions. The spectrum of clinical manifestations... Read more

Industry

view channel
Image: For 46 years, Roche and Hitachi have collaborated to deliver innovative diagnostic solutions (Photo courtesy of Roche)

Roche and Hitachi High-Tech Extend 46-Year Partnership for Breakthroughs in Diagnostic Testing

Roche (Basel, Switzerland) and Hitachi High-Tech (Tokyo, Japan) have renewed their collaboration agreement, committing to a further 10 years of partnership. This extension brings together their long-standing... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.