We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
16 Aug 2021 - 26 Aug 2021
Virtual Venue

Early-Detection Urine Test Is Effective for Lyme Disease

By LabMedica International staff writers
Posted on 01 Jun 2016
Print article
Image: The typical erythema migrans or bulls-eye rash often but not always seen in Lyme disease (Photo courtesy of the CDC).
Image: The typical erythema migrans or bulls-eye rash often but not always seen in Lyme disease (Photo courtesy of the CDC).
Prompt antibiotic treatment of early stage Lyme Borreliosis (LB) prevents progression to severe multisystem disease and there is a clinical need to improve the diagnostic specificity of early stage Lyme assays in the period prior to the mounting of a robust serology response.

The clinical presentation of LB can be so varied; early stage disease can be misdiagnosed for a variety of reasons including failure to develop an erythema migrans (EM) rash, failure of the patient or clinician to recognize an EM rash, if present, the non-specific nature of early symptoms and a negative or ambiguous serology.

Scientists at the George Mason University (Fairfax, VA, USA) obtained mid-stream urine specimens that were self-collected by 268 participants with all stages of documented or suspected Lyme disease. Whole blood from each subject was drawn on the same day as urine collection. Blood samples were sent to Quest Diagnostics (Madison, NJ, USA) for Total Lyme Disease Antibody (EIA) and Lyme immunoglobulin G (IgG) and IgM Western Blot tests.

The scientists employed Nanotrap particles (Ceres Nanosciences, Manassas, VA, USA) to concentrate urinary OspA and used a highly specific anti-OspA monoclonal antibody (mAb) as a detector of the C-terminus peptides. They mapped the mAb epitope to a narrow specific OspA C-terminal domain OspA236-239 conserved across infectious Borrelia species but with no homology to human proteins and no cross-reactivity with relevant viral and non-Borrelia bacterial proteins. They collected 268 urine samples from patients being evaluated for all categories of LB in a LB endemic area. The urinary OspA assay, blinded to outcome, utilized Nanotrap particle pre-processing, western blotting to evaluate the OspA molecular size, and OspA peptide competition for confirmation.

The team found that in pre-treatment, 24/24 newly diagnosed patients with an erythema migrans (EM) rash were positive for urinary OspA while false positives for asymptomatic patients were 0/117. For 10 patients who exhibited persistence of the EM rash during the course of antibiotic therapy, 10/10 were positive for urinary OspA. Urinary OspA of 8/8 patients switched from detectable to undetectable following symptom resolution post-treatment. Specificity of the urinary OspA test for the clinical symptoms was 40/40. Specificity of the urinary OspA antigen test for later serology outcome was 87.5 % (21 urinary OspA positive/24 serology positive, and 41 of 100 patients under surveillance for persistent LB in an endemic area were positive for urinary OspA protein.

Alessandra Luchini, PhD, a professor and lead investigator of the study, said, “We're looking to repeat the story again with these other diseases. Other targets for the new type of test include Chagas disease, which is infectious and caused by a parasite, and toxoplasmosis, another parasite-borne disease.” The study was published originally published online on November 4, 2015, in the Journal of Translational Medicine.

Related Links:
George Mason University
Quest Diagnostics
Ceres Nanosciences
Gold Supplier
Molecular Diagnostics System
ePlex System
Digital Test Reader
SARS-CoV-2 Detection Kit
Loopamp SARS-CoV-2 Detection Kit

Print article


Molecular Diagnostics

view channel
Image: The Lumipulse G β-amyloid 1-40 assay kit (Photo courtesy Fujirebio)

APOE Gene Influences Alzheimer's Symptoms, Biomarkers in Down Syndrome

Individuals with Down syndrome (DS) constitute a population at ultrahigh risk of developing Alzheimer disease (AD) because of trisomy of chromosome 21, which harbors the amyloid precursor protein (APP) gene.... Read more


view channel

ELITechGroup Acquires Freezing Point Osmometry Provider GONOTEC

ELITechGroup (Puteaux, France) has acquired GONOTEC (Berlin, Germany), thus uniting two osmometry market leaders. The acquisition will also provides the industry with a full range of osmometry solutions... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.