We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Urine Test Detects Parkinson’s by Identifying Protein from Brain Cells

By LabMedica International staff writers
Posted on 18 May 2023
Print article
Image: The EVtrap technology uses magnetic beads to rapidly isolate and identify large quantities of proteins from extracellular vesicles (Photo courtesy of Purdue University)
Image: The EVtrap technology uses magnetic beads to rapidly isolate and identify large quantities of proteins from extracellular vesicles (Photo courtesy of Purdue University)

It's estimated that 1% of individuals aged over 60 are affected by Parkinson's disease. Unfortunately, diagnosing this kind of neurodegenerative disease is challenging, with cognitive and movement tests sometimes taking over a year to confirm the diagnosis. Early diagnostic molecular tests could speed up interventions and help Parkinson's patients receive treatment faster. Now, researchers have devised a new technique that can reveal signs of Parkinson’s disease in urine samples.

Researchers at Purdue University (West Lafayette, IN, USA) developed a method that potentially enables the detection of alterations in LRRK2 (leucine-rich repeat kinase 2) proteins and their downstream pathways in urine samples of Parkinson's patients. LRRK2 proteins are known to be associated with Parkinson's disease. This innovative approach might also pave the way for noninvasive testing for other neurodegenerative disorders and cancers. Among several methods to study the effect of LRRK2, tracking its biological pathway is feasible through analysis of urine, blood, and cerebrospinal fluid.

Extracellular vesicles (EVs), minute packages utilized by cells for molecular delivery, are present in phosphorylated proteins that are common indicators of cancers. Previous studies suggested that blood samples with phosphoproteins could be potential markers for early cancer detection or disease progression monitoring. EVs offer a way to target disease markers, as they are released by specific types of cells. However, sampling such biomarkers from the brain via spinal tap is a highly intrusive process.

The potential of urine as a source of brain-related chemical markers was previously unknown. Although urine samples contain proteins that might serve as disease markers, many are involved in general cell maintenance and unrelated to diseases. Interestingly, these EVs can readily cross the blood-brain barrier. Upon being discharged from the brain into the bloodstream, they become concentrated or filtered into the urine. In this research, the team successfully isolated these EVs quickly from urine samples, using the EVtrap (Extracellular Vesicles total recovery and purification) method. The EVtrap method offers a simple way to monitor changes in urine, which is routinely collected in various clinical studies.

“It’s going to be a big new area in diagnostic development, especially for neurodegenerative diseases and cancer,” predicted co-author Anton Iliuk. “This kind of analysis opens a new frontier in noninvasive diagnostics development. It’s showing that biomarkers previously thought to be undetectable have become uncovered and do a really good job of differentiating disease from non-disease state.”

Related Links:
Purdue University 

Flocked Swab
HydraFlock and PurFlock Ultra
Gold Supplier
STI Real-Time PCR Test
Neisseria Gonorrhoeae/Chlamydia Trachomatis/U.urealyticum Real-Time PCR Kit
Lung Cancer Molecular Diagnostic Test
Semi-Automatic Coagulation Analyzer
Clot 2B

Print article


Clinical Chem.

view channel
Image: Electrochemical cells etched by laser on wooden tongue depressor measure glucose and nitrite in saliva (Photo courtesy of Analytical Chemistry)

Biosensor-Fabricated Wooden Tongue Depressor Measures Glucose and Nitrite in Saliva

Physicians often use tongue depressors to examine a patient's mouth and throat. However, it is hard to imagine that this simple wooden tool could actively assess a patient's health. This idea has led to... Read more


view channel
Image: The Atellica HEMA 570 and 580 hematology analyzers remove workflow barriers (Photo courtesy of Siemens)

Next-Gen Hematology Analyzers Eliminate Workflow Roadblocks and Achieve Fast Throughput

Hematology testing is a critical aspect of patient care, utilized to establish a patient's health baseline, track treatment progress, or guide timely modifications to care. However, increasing constraints... Read more


view channel
Image: Newly observed anti-FSP antibodies have also been found to predict immune-related adverse events (Photo courtesy of Calviri)

First Blood-Based Biomarkers Test to Predict Treatment Response in Cancer Patients

Every year worldwide, lung cancer afflicts over two million individuals and almost the same number of people succumb to the disease. This malignancy leads the charts in cancer-related mortalities, with... Read more


view channel
Image: The rapid MTB strip test for tuberculosis can identify TB patients within two hours (Photo courtesy of Chulalongkorn University)

Rapid MTB Strip Test Detects Tuberculosis in Less Than an Hour without Special Tools

Tuberculosis (TB), a highly infectious disease, continues to pose significant challenges to public health worldwide. TB is caused by a bacterium known as "Mycobacterium tuberculosis," spreading through... Read more


view channel
Image: The UNIQO 160 (CE-IVDR) advances diagnostic analysis for autoimmune diseases (Photo courtesy of EUROIMMUN)

Novel Automated IIFT System Enables Cutting-Edge Diagnostic Analysis

A newly-launched automated indirect immunofluorescence test (IIFT) system for autoimmune disease diagnostics offers an all-in-one solution to enhance the efficiency of the complete IIFT process, comprising... Read more


view channel
Electronic biosensor uses DNA aptamers for detecting biomarkers in whole blood samples (Photo courtesy of Freepik)

Electronic Biosensor Detects Biomarkers in Whole Blood Samples without Addition of Reagents

The absence of robust, reliable, and user-friendly bioanalytical tools for early and timely diagnosis of cardiovascular diseases, particularly sudden cardiac arrest, leads to preventable deaths and imposes... Read more


view channel
Image: The global hemostasis diagnostics market is expected to reach USD 3.95 billion by 2025 (Photo courtesy of Freepik)

Global Hemostasis Diagnostics Market Driven by Increase in Invasive Surgical Procedures

Injury or surgery naturally creates bleeding in living beings, which must be stopped to prevent excessive blood loss. The human body implements a protective mechanism known as hemostasis to stop excessive bleeding.... Read more
Copyright © 2000-2023 Globetech Media. All rights reserved.