We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics

AGILENT

  Silver Agilent provides laboratories worldwide with instruments, services, consumables, applications and expertise, enabling... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
23 Sep 2021 - 25 Sep 2021

Various Serum Vitamin D Biomarkers Compared for Sarcopenia

By LabMedica International staff writers
Posted on 25 Aug 2021
Print article
Image: The Agilent 1260 HPLC system (Photo courtesy of Agilent Technologies)
Image: The Agilent 1260 HPLC system (Photo courtesy of Agilent Technologies)
Sarcopenia is an age-related clinical condition characterized by a gradual and generalized loss of skeletal muscle mass with a decrease in strength and physical capacity. It is considered to be one of the risk factors for adverse events in older people, including delirium, disability, institutionalization, and even death.

Vitamin D deficiency is common among older people around the world. Older people are particularly prone to the development of vitamin D insufficiency or deficiency for following reasons: a reduced cutaneous synthesis in the skin, decreased daily sun exposure, and chronic diseases of organs related to vitamin D metabolism.

Clinical Laboratorians at the Gyeongsang National University College of Medicine (Jinju, Korea) and their medical colleagues collected data from May 2018 to December 2019 on a total of 83 hip fracture (HF) surgery patients: 36 with sarcopenia and 47 non-sarcopenia patients, who were included in the analysis. The age of patients was 74.1 ± 12.3 years in the sarcopenia group and 70.7 ± 10.0 years in the non-sarcopenia group. The male-to-female ratio was 0.59 in the sarcopenia group and 0.33 in the non-sarcopenia group.

Measurements of bioavailable 25(OH)D, 24,25-dihydroxyvitamin D (24,25(OH)2D) and total 25-hydroxy vitamin D (25(OH)D) were made using solid-phase extraction (SPE) and subsequent liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS system consisted of an Agilent 1260 HPLC system with an Agilent 6460 triple quadrupole mass spectrometer (Agilent Technologies, Santa Clara, CA, USA).

Vitamin D binding protein (VDBP) concentration was measured using an enzyme-linked immunosorbent assay (R&D Systems, Minneapolis, MN., USA). For GC gene genotyping, genomic DNA was isolated from peripheral blood leukocytes and performed using a TaqMan SNP Genotyping Assay and an ABI ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).

The investigators reported that bioavailable 25(OH)D levels were significantly decreased in the sarcopenia group compared with the non-sarcopenia group. Results of ROC analysis for the diagnosis of sarcopenia using serum level of bioavailable of 25(OH)D revealed that the cutoff point for bioavailable 25(OH)D was 1.70 ng/mL (AUC = 0.649). In the group with a bioavailable 25(OH)D less than 1.70 ng/mL, the incidence of sarcopenia increased by 3.3 times. Among laboratory test results, serum levels of albumin, calcium, alkaline phosphatase, aspartate aminotransferase (AST), and alanine aminotransferase (AST) were not significantly different either between the two groups (sarcopenia versus non-sarcopenia). However, parathyroid hormone (PTH) was significantly higher in the non-sarcopenia group than in the sarcopenia group.

The authors concluded that among various serum vitamin D biomarkers, bioavailable 25(OH)D was associated with sarcopenia. Thus, bioavailable vitamin D might be used as an auxiliary marker for assessing the risk of sarcopenia. The study was published on August 5, 2021 in the Journal of Clinical Laboratory Analysis.

Related Links:
Gyeongsang National University College of Medicine
Agilent Technologies
R&D Systems
Applied Biosystems


New
Gold Supplier
Rapid PCR Diagnostic System
Accula System
New
Laboratory Information System (LIS)
GLIMS 10
New
Silver Supplier
COVID-19 NAb Test Solution
COVID-19 NAb Test Solution
New
Silver Supplier
Automated Nucleic Acid Extractor
NE48/96

Print article

Channels

Pathology

view channel
Image: The CellSearch Circulating Tumor Cell Kit is intended for the enumeration of circulating tumor cells of epithelial origin (CD45-, EpCAM+, and cytokeratins 8, 18+, and/or 19+ and PD-L1) in whole blood (Photo courtesy of CellSearch/Menarini Silicon Biosystems)

PD-L1 Expression in Circulating Tumor Cells Investigated for NSCLC

In non-small cell lung cancer (NSCLC), analysis of programmed cell death ligand 1 (PD-L1) expression in circulating tumor cells (CTCs) is a potential alternative to overcome the problems linked to the... Read more

Industry

view channel
Illustration

Global Digital Polymerase Chain Reaction (dPCR) Market Projected to Reach Close to USD 1.15 Billion by 2028

The global digital polymerase chain reaction (dPCR) market is projected to grow at a CAGR of more than 9% from over USD 0.50 billion in 2020 to nearly USD 1.15 billion by 2028, driven primarily by rising... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.