We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

QIAGEN

Qiagen is a provider of sample and assay technologies for molecular diagnostics and applied testing, including comple... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Fecal Immunochemical Tubes Sourced to Analyze Gut Microbiome for CRC

By LabMedica International staff writers
Posted on 14 Oct 2021
Print article
Image: The QuikRead go iFOBT is an immunochemical fecal immunochemical test for detection and quantification of human hemoglobin in feces in case of suspected bleeding from the lower gastrointestinal tract (Photo courtesy of Aidian)
Image: The QuikRead go iFOBT is an immunochemical fecal immunochemical test for detection and quantification of human hemoglobin in feces in case of suspected bleeding from the lower gastrointestinal tract (Photo courtesy of Aidian)
Colorectal cancer (CRC) is a challenging public health problem which successful treatment depends on the stage at diagnosis. Recently, CRC-specific microbiome signatures have been proposed as a marker for CRC detection.

Accumulating evidence indicates that the microbiome substantially contributes to the development of CRC. Cross-sectional multi-population studies in humans in which the CRC microbiome and cancer stage-specific microbial signatures have been detected in the stool samples of CRC patients have shown significant associations between the gut microbiota and CRC.

Genomic Scientists at the University of Tartu (Tartu, Estonia) enrolled 30 volunteers (16 women and 14 men) without known gastrointestinal disorders and who had not taken antibiotics within 60 days prior to sampling. All recruited subjects were Estonians aged between 22 and 68 (39 ± 12.1) years. Seven fecal samples were self-collected by each volunteer, and 214 samples (including positive and negative controls) were analyzed. From each fecal sample, three subsamples from each individual were transferred to QuikRead go iFOB fecal immunochemical test tubes (Aidian, Espoo, Finland).

DNA extraction from all samples was performed using a Qiagen DNeasy PowerSoil Pro DNA extraction kit (Qiagen, Venlo, The Netherlands). Amplicon libraries for Illumina (Illumina, San Diego, CA, USA) next-generation sequencing were generated by two-step PCR. The quality control of the amplicon libraries was performed via Agilent 2200 TapeStation analysis (Agilent Technologies, Santa Clara, CA, USA). Sequencing was carried out on an Illumina MiSeq System.

The investigators reported that all genera expected to be present in the positive controls (Staphylococcus, Pseudomonas, Enterococcus, Escherichia, Salmonella, Lactobacillus, Listeria, and Bacillus) were detected in the analysis. As expected, a Western microbial community structure was observed in all of the sample types, with 90% of bacteria belonging to the phyla Firmicutes and Bacteroides, followed by the phyla Proteobacteria, Actinobacteria, and Verrucomicrobia. The results indicated that the variation between individuals was greater than the differences introduced by the collection strategy. The vast majority of the genera were stable for up to seven days. None of the changes observed between fresh-frozen samples and FIT tube specimens were related to previously identified CRC-specific bacteria.

Kertu Liis Krigul, PhD, a Molecular Geneticist and first author of the study, said, “Current screening programs, however, face multiple challenges, including low participation rates, low sensitivity for pre-cancerous or early-stage cancer, and false positive and false-negative results among others. Consequently, new highly specific, inexpensive, and sensitive noninvasive screening tests that improve the detection of precancerous colorectal lesions and cancer are urgently needed to reduce the incidence and mortality of the disease.”

The authors concluded that their results showed for the first time that QuikRead iFOB sampling set tubes are suitable for storing fecal samples for microbiome studies, as the captured microbiome profile is similar to that obtained from fresh-frozen samples and remains stable for up to seven days. The study was published on October 1, 2021 in the journal Scientific Reports.

Related Links:
University of Tartu
Aidian
Qiagen
Illumina
Agilent Technologies


Gold Supplier
SARS-CoV-2 Multiplex Real-Time RT-PCR Assay
GSD NovaPrime Plus SARS-CoV-2
New
6-Channel Viscoelastometry Analyzer
ClotPro
New
SARS-CoV-2 Antigen Immunofluorescence System
Watmind SARS-CoV-2 Antigen Immunofluorescence System
New
Tuberculosis (TB) Test
QIAreach QuantiFERON-TB

Print article

Channels

Hematology

view channel
Image: Atellica Solution (Photo courtesy of Siemens Healthineers)

Siemens Introduces New Intelligent, Integrated IVD Solutions Virtually at EUROMEDLAB 2021

Siemens Healthineers (Erlangen, Germany) introduced new intelligent, integrated IVD solutions virtually at the XXIV IFCCEFLM European Congress of Clinical Chemistry and Laboratory Medicine (EuroMedLab... Read more

Immunology

view channel
Image: The Luminex 200 Instrument System sets the standard for multiplexing, providing the ability to perform up to 100 different tests in a single reaction volume on a flow cytometry-based platform (Photo courtesy of Luminex Corp)

Inflammatory Cytokines Measured in Infants Born to Preterm Preeclamptic Mothers

Preeclampsia is both a vascular and inflammatory disorder. The pathophysiology of preeclampsia is complex and rooted in the interplay between maternal and placental factors with the key characteristics... Read more

Microbiology

view channel
Image: The sciREADER CL2 enables high quality digital colorimetric imaging of various support formats (Photo courtesy of SCIENION)

Multiplex Immunoassay Developed for Confirmation and Typing of HTLV Infections

Human T-Cell Lymphotropic Viruses (HTLV) type 1 and type 2 account for an estimated five to 10 million infections worldwide and are transmitted through breast feeding, sexual contacts and contaminated... Read more

Pathology

view channel
Image: The Ventana BenchMark Ultra autostainer is for cancer diagnostics with automation and the test menu include IHC, ISH, and FITC tests (Photo courtesy of Ventana Medical System)

Specific Biomarker Investigated for Triple-Negative Breast Cancer Diagnosis

Triple-negative breast cancer (TNBC) is defined by the lack of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) expression and comprises a heterogeneous... Read more

Technology

view channel
Image: PKeye Workflow Monitor System (Photo courtesy of PerkinElmer, Inc.)

PerkinElmer’s New Cloud-Based Platform Enables Laboratory Personnel to Remotely Manage Its Instruments in Real-Time

PerkinElmer, Inc. (Waltham, MA; USA) has launched its PKeye Workflow Monitor, a cloud-based platform enabling laboratory personnel to remotely manage and monitor their PerkinElmer instruments and workflows... Read more

Industry

view channel
Illustration

Global Lateral Flow Assay Market to Reach Nearly USD 6.5 Billion by 2031 Due to Surge in Demand for Rapid POC Testing

The global lateral flow assay market is projected to grow at a CAGR of around 5% from USD 3.7 billion in 2020 to over USD 6.4 billion by 2031, driven by the growing adoption of home-based lateral flow... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.