We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

Download Mobile App




Integrated Solution for Rapid AST Directly From Positive Blood Cultures to Combat Bloodstream Infection

By LabMedica International staff writers
Posted on 29 Sep 2023
Print article
Image: On-chip pretreatment and rapid AST based directly on positive blood cultures (Photo courtesy of Liu Yang)
Image: On-chip pretreatment and rapid AST based directly on positive blood cultures (Photo courtesy of Liu Yang)

The presence of living bacteria in the bloodstream, known as bacteremia, can lead to serious conditions like bloodstream infections (BSIs) and sepsis, which can often be fatal. Quickly prescribing the right antibiotics is crucial for reducing the risk of death in patients with BSIs. Traditional methods for antimicrobial susceptibility testing (AST) to guide these prescriptions can take a long time, leaving doctors to depend mostly on their own expertise. To tackle this issue, researchers have come up with a specialized microfluidic chip called the BSI-AST chip. This chip can extract bacteria and perform AST directly from positive blood cultures in under 3.5 hours, offering a faster and more effective approach to managing BSIs.

Existing AST techniques take a minimum of two days to provide results after a blood culture tests positive for bacteria. This delay pushes healthcare providers to prescribe broad-spectrum antibiotics without waiting for test results, which could worsen the patient's condition and contribute to antibiotic resistance. In response to this urgent need, a group of researchers, including scientists from the Chinese Academy of Sciences (CAS, Beijing, China), have developed the BSI-AST chip. This chip is capable of isolating bacteria from a positive blood culture in just 10 minutes, and completing the AST in an additional three hours. In a proof-of-concept experiment, the chip successfully performed AST on artificial positive blood cultures containing E. coli, and was tested against 18 different antibiotics, all within less than 3.5 hours.

The BSI-AST chip was also tested with actual clinical positive blood cultures and showed a 93.3% agreement with conventional clinical testing methods. This chip's quick and reliable results demonstrate its immense potential for use in clinical settings. What sets this chip apart is that it can operate directly on positive blood cultures without needing an additional subculture step. This is achieved through the introduction of a separating gel into the microfluidic chip for the first time. Centrifugal microfluidic enrichment technology also played a key role. Additionally, the chip can perform multiple analyses simultaneously through antibiotic drying and parallel array techniques, helping doctors make better antibiotic choices for their BSI patients. The chip can also be easily integrated with Clinical Antimicrobial Susceptibility Test Ramanometry (CAST-R), another invention from the same team, to provide a streamlined solution for sample pretreatment.

"Rapid AST in blood culture is significant for patients with clinical sepsis and has the potential to save lives," said Prof. Cheng Yongqiang, the study's corresponding author, who also noted the role of such technology in combating the serious threat of microbial resistance to humanity.

Related Links:
CAS 

New
Platinum Member
Flu SARS-CoV-2 Combo Test
OSOM® Flu SARS-CoV-2 Combo Test
Magnetic Bead Separation Modules
MAG and HEATMAG
POCT Fluorescent Immunoassay Analyzer
FIA Go
Gold Member
Liquid Ready-To-Use Lp(a) Reagent
Lipoprotein (a) Reagent

Print article
77 ELEKTRONIKA

Channels

Clinical Chemistry

view channel
Image: PhD student and first author Tarek Eissa has analyzed thousands of molecular fingerprints (Photo courtesy of Thorsten Naeser / MPQ / Attoworld)

Screening Tool Detects Multiple Health Conditions from Single Blood Drop

Infrared spectroscopy, a method using infrared light to study the molecular composition of substances, has been a foundational tool in chemistry for decades, functioning similarly to a molecular fingerprinting... Read more

Molecular Diagnostics

view channel
Image: Protein ‘signatures’ obtained via a blood sample can be used to predict the onset of 67 diseases (Photo courtesy of Queen Mary University of London)

Protein Signatures in Blood Can Predict Risk of Developing More Than 60 Diseases

Measuring specific proteins to diagnose conditions like heart attacks, where troponin is tested, is a well-established clinical practice. Now, new research highlights the broader potential of protein measurements... Read more

Hematology

view channel
Image: The Truvian diagnostic platform combines clinical chemistry, immunoassay and hematology testing in a single run (Photo courtesy of Truvian Health)

Automated Benchtop System to Bring Blood Testing To Anyone, Anywhere

Almost all medical decisions are dependent upon laboratory test results, which are essential for disease prevention and the management of chronic illnesses. However, routine blood testing remains limited worldwide.... Read more

Pathology

view channel
Image: The tool can improve precision oncology by accurately predicting molecular subtypes and therapy responses (Photo courtesy of Shutterstock)

Computational Tool Integrates Transcriptomic Data for Improved Breast Cancer Diagnosis and Treatment

Breast cancer is the most commonly diagnosed cancer globally, presenting in various subtypes that require precise identification for effective, personalized treatment. Traditionally, cancer subtyping has... Read more

Industry

view channel
Image: Beckman Coulter will utilize the ALZpath pTau217 antibody to detect key biomarker for Alzheimer\'s disease on its DxI 9000 immunoassay analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter Licenses Alzpath's Proprietary P-tau 217 Antibody to Develop Alzheimer's Blood Test

Cognitive assessments have traditionally been the primary method for diagnosing Alzheimer’s disease, but this approach has its limitations as symptoms become apparent only after significant brain changes... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.