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监测查加斯病心肌病的试剂

By LabMedica International staff writers
Posted on 22 Apr 2011
血液中一种蛋白质的血清测定已被用于监测慢性查加斯病患者的心脏损伤。

一种测量蛋白生物标记物——心肌肌钙蛋白T (cTnT)的高灵敏测定已被用于评估克氏锥虫(一种血液传播的原生动物寄生虫)所致热带病患者的心脏损伤。

德国柏林的Charité大学医院(www.charite.de)开展了一项国际性研究,测量了26名健康受试者和179名慢性查加斯病患者的血清cTnT,这些患者中86人无症状,71人有心肌病且伴有巨结肠症或者不伴有巨结肠症,22人只有巨结肠症。巨结肠症是结肠的异常膨大,常伴有肠蠕动的瘫痪。

研究人员分别用传统的第四代测定和罗氏诊断公司(Mannheim, Germany; www.roche.com)的高灵敏测定在罗氏Elecsys-2010化学分析仪上测量了血清cTnT。无论有无巨结肠症,心肌病患者的血清cTnT水平明显高于健康受试者、无症状患者和巨结肠症患者,且cTnT的测量值与心肌病的严重程度相关。这种高灵敏测定的检测下限是3 ng/L,是区分心脏损伤患者与未损伤患者的理想手段。不同类型的查加斯病患者和严重程度不同的心肌病患者cTnT水平的变化都伴有C反应蛋白和白细胞介素6。

该研究的论文发表于2011年2月的《病理与医疗化验文集》(Archives of Pathology and Laboratory Medicine)杂志。作者评论说,灵敏度更高的心肌肌钙蛋白免疫测定能反映心肌肌钙蛋白水平微量但却明确的升高,对于心脏的病理生理病症有诊断和预后作用,如对炎症和心肌病。这种高灵敏的cTnT测定是一种慢性查加斯病的诊断工具,它提供的数据可用于心肌病的诊断、预后和监测。他们认为,cTnT测量可对心电图、超声心动图和放射成像起到补充作用,在特殊情况下还可先于这些诊断工具使用。

Related Links:
Charité University Hospital >>> www.charite.de
Roche Diagnostics >>> www.roche.com
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