We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

Download Mobile App




Novel LC-MS/MS Assay Detects Low Creatinine in Sweat and Saliva

By LabMedica International staff writers
Posted on 01 Jan 2025
Print article
Image: The LC-MS/MS assay can serve as a reference method to validate emerging biosensors for renal function monitoring (Photo courtesy of 123RF)
Image: The LC-MS/MS assay can serve as a reference method to validate emerging biosensors for renal function monitoring (Photo courtesy of 123RF)

Timely and accurate monitoring of renal function is essential for managing patients at risk of acute kidney injury (AKI), which affects about 12% of hospitalized patients and up to 57% of ICU patients. In approximately 20% of these cases, the condition progresses to more severe stages of AKI, leading to longer hospital stays, higher mortality rates, and an increased need for invasive renal replacement therapies. Early intervention, driven by frequent or continuous monitoring of renal function biomarkers like creatinine, could potentially delay this progression. As renal failure can advance quickly, frequent venipuncture for creatinine testing is required. Recent advances in microfluidic devices and biosensors have led to the exploration of alternative biofluids, such as sweat and saliva, for non-invasive, continuous monitoring of creatinine. Researchers have now developed a novel Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) assay for detecting low creatinine levels in these biofluids.

Researchers at Eindhoven University of Technology (Eindhoven, The Netherlandsl) investigated the correlation between creatinine concentrations in sweat, saliva, and plasma during hemodialysis, with an eye toward future biosensor applications. They measured creatinine levels in sweat, saliva, and plasma from 40 patients undergoing hemodialysis. Using the novel LC-MS/MS assay, they quantified low creatinine concentrations in these biofluids. Correlation analyses were conducted to compare creatinine levels across the biofluids. The LC-MS/MS assay demonstrated excellent accuracy (93.9% to 97.8%) and low imprecision (3.4% to 8.1%) for measuring very low creatinine concentrations, with a limit of quantitation of 1.26 µmol/L.

The study revealed strong correlations between creatinine concentrations in sweat and saliva compared to plasma (ρ: 0.68 and 0.80, respectively). During hemodialysis, creatinine concentrations declined similarly across all three biofluids. These strong correlations suggest that sweat and saliva could serve as reliable, non-invasive alternatives to traditional blood tests for assessing kidney function. This research improves the understanding of creatinine excretion pathways and lays the groundwork for the development of patient-friendly, continuous kidney function monitoring methods, such as wearable biosensors.

Gold Member
Troponin T QC
Troponin T Quality Control
Verification Panels for Assay Development & QC
Seroconversion Panels
New
Chagas Disease Test
LIAISON Chagas
New
HbA1c Test
HbA1c Rapid Test

Print article

Channels

Molecular Diagnostics

view channel
Image: Health Canada has approved SPINEstat, a first-in-class diagnostic blood test for axSpA, as a Class II medical device (Photo courtesy of Augurex)

First-in-Class Diagnostic Blood Test Detects Axial Spondyloarthritis

Axial spondyloarthritis (axSpA) is a chronic inflammatory autoimmune condition that typically affects individuals during their most productive years, with symptoms often emerging before the age of 45.... Read more

Immunology

view channel
Image: The cancer stem cell test can accurately choose more effective treatments (Photo courtesy of University of Cincinnati)

Stem Cell Test Predicts Treatment Outcome for Patients with Platinum-Resistant Ovarian Cancer

Epithelial ovarian cancer frequently responds to chemotherapy initially, but eventually, the tumor develops resistance to the therapy, leading to regrowth. This resistance is partially due to the activation... Read more

Technology

view channel
Image: The new algorithms can help predict which patients have undiagnosed cancer (Photo courtesy of Adobe Stock)

Advanced Predictive Algorithms Identify Patients Having Undiagnosed Cancer

Two newly developed advanced predictive algorithms leverage a person’s health conditions and basic blood test results to accurately predict the likelihood of having an undiagnosed cancer, including ch... Read more

Industry

view channel
Image: The collaboration aims to leverage Oxford Nanopore\'s sequencing platform and Cepheid\'s GeneXpert system to advance the field of sequencing for infectious diseases (Photo courtesy of Cepheid)

Cepheid and Oxford Nanopore Technologies Partner on Advancing Automated Sequencing-Based Solutions

Cepheid (Sunnyvale, CA, USA), a leading molecular diagnostics company, and Oxford Nanopore Technologies (Oxford, UK), the company behind a new generation of sequencing-based molecular analysis technologies,... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.