We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Holographic Histopathology Enables Fast, Precise Diagnostics

By LabMedica International staff writers
Posted on 10 May 2021
Print article
Image: Schematic of the imaging of pathological tissue 3D structure by combining optical diffraction tomography and automated stitching (Photo courtesy of Korea Advanced Institute of Science and Technology)
Image: Schematic of the imaging of pathological tissue 3D structure by combining optical diffraction tomography and automated stitching (Photo courtesy of Korea Advanced Institute of Science and Technology)
Microscopic assessment of biopsied and resected tissues is central to understanding the underlying pathophysiology and clinical states of many patients. Because most microscopic specimens are translucent to visible light, conventional histological methods achieve image contrast using exogenous labels.

Optical diffraction tomography is a microscopy technique for reconstructing the refractive index of a tissue sample from its scattered field images obtained with various illumination angles. It enables label-free high contrast visualization of transparent samples. The complex scattered field transmitted through the sample is first retrieved using off-axis holography, then the scattered fields obtained with various angle of illuminations are mapped in the Fourier space enabling the reconstruction of the sample refractive index.

Bioscientists at the Korea Advanced Institute of Science and Technology (Daejeon, Republic of Korea) and their colleagues developed label-free volumetric imaging of thick-tissue slides, exploiting refractive index distributions as intrinsic imaging contrast. The present method systematically exploited label-free quantitative phase imaging techniques, volumetric reconstruction of intrinsic refractive index distributions in tissues, and numerical algorithms for the seamless stitching of multiple three-dimensional tomograms and for reducing scattering-induced image distortion.

The scientists demonstrated label-free volumetric imaging of thick tissues with the field of view of 2  mm  ×  1.75  mm  ×  0.2  mm with a spatial resolution of 170  nm  ×  170  nm  ×  1,400  nm. The number of optical modes, calculated as the reconstructed volume divided by the size of the point spread function, was ∼20  giga voxels. They have also demonstrated that different tumor types and a variety of precursor lesions and pathologies can be visualized with the present method.

The team demonstrated the capacity of their novel method by imaging a variety of different cancer pathologies: pancreatic neuroendocrine tumor, intraepithelial neoplasia, and intraductal papillary neoplasm of bile duct. They imaged millimeter-scale, unstained, 100-μm-thick tissues at a subcellular 3D resolution, which enabled the visualization of individual cells and multicellular tissue architectures, comparable to images obtained with traditional chemically processed tissues.

YongKuen Park, PhD, a professor and senior author of the study, said, “The images obtained with the proposed method enabled clear visualization of different morphological features in the various tissues allowing for recognition and diagnosis of precursor lesions and pathologies. We expect optical diffraction tomography to provide faster and more precise diagnostics in histopathology and intraoperative pathology consultations.” The study was published on April 29, 2021 in the journal Advanced Photonics.

Related Links:
Korea Advanced Institute of Science and Technology

New
Gold Supplier
COVID-19 Real Time RT-PCR Kit
respiraScreen 1
New
Silver Supplier
COVID-19 NAb Test Solution
COVID-19 NAb Test Solution
New
SARS-CoV-2 Antigen Rapid Test
Flowflex SARS-CoV-2 Antigen Rapid Test (Nasal/Saliva)
New
Gold Supplier
SARS-CoV-2 Multiplex Real-Time RT-PCR Assay
GSD NovaPrime Plus SARS-CoV-2

Print article

Channels

Pathology

view channel

Certest Offers Real-Time PCR Assays for Fast Detection of MDR Bacterial Infections

CerTest Biotec (Zaragoza, Spain) has joined the fight against multidrug resistant strains (MDR) bacterial infections by developing real-time PCR assays for the fast detection of genes or punctual mutations that confer resistance to antibiotics from both Gram-positive and Gram-negative pathogens. Resistance to antibiotics... Read more

Industry

view channel
Illustration

Thermo Fisher Launches World’s First Fully Integrated Digital PCR (dPCR) System

Thermo Fisher Scientific Inc. (Waltham, MA, USA) has launched the world’s first fully integrated digital PCR (dPCR) system designed to provide highly accurate and consistent results within 90 minutes.... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.