Bioelectronic Device Detects Marker Linked to Neurodegenerative Diseases and Cancer

Their first biosensor successfully detects the enzyme glutathione S-transferase (GST), linked to Parkinson's, Alzheimer's and breast cancer, among other diseases. The team is also developing an innovative paper-based electronic sensor to lower the cost even further.

The biosensor, developed by researchers at Brazil’s National Nanotechnology Laboratory (LNNano; Campinas, São Paulo State, Brazil), is basically a single-layer organic nanometer-scale transistor on a glass slide. It contains the reduced form of the peptide glutathione (GSH), which reacts upon contact with disease marker GST. The GSH-GST reaction is detected by the transistor, which can be used for diagnostic purposes.

The device was developed as part of a project that focuses on development of point-of-care (POC) devices by researchers in a range of knowledge areas, using functional materials to produce simple sensors and microfluidic systems for rapid diagnosis.

"Platforms like this one can be deployed to diagnose complex diseases quickly, safely, and relatively cheaply, using nanometer-scale systems to identify molecules of interest in the material analyzed," explained Carlos Cesar Bof Bufon, head of LNNano's Functional Devices & Systems Lab (DSF) and member of the project, whose principal investigator is Lauro Kubota, professor, Chemistry Institute (IQ-UNICAMP), University of Campinas.

In addition to portability and low cost, the advantages of the nanometric biosensor include its detection sensitivity. "This is the first time organic transistor technology has been used in detecting the pair GSH-GST, which is important in diagnosing degenerative diseases, for example," explained Prof. Bufon, "The device can detect such molecules even when they're present at very low levels in the examined material, thanks to its nanometric sensitivity."

The system can be adapted to detect other substances, such as molecules linked to other diseases, and elements present in contaminated material, among other applications. This requires replacing the molecules in the sensor with others that react with the analytes targeted by the test.

The team is working on paper-based biosensors to further lower cost, improve portability, and facilitate fabrication as well as disposal. The challenge is that paper is an insulator in its usual form. Prof. Bufon has developed a technique to make paper conductive and capable of transporting sensing data by impregnating cellulose fibers with polymers that have conductive properties. The technique is based on in situ synthesis of conductive polymers. For the polymers not to remain trapped on the surface of the paper, they have to be synthesized inside and between the pores of the cellulose fibers.

This is achieved by gas-phase chemical polymerization: a liquid oxidant is infiltrated into the paper, which is then exposed to monomers in the gas phase. The monomers evaporate under the paper and penetrate the pores of the fibers at the submicrometer scale. Inside the pores, they blend with the oxidant and begin the polymerization process right there, impregnating the entire material. The polymerized paper acquires the conductive properties of the polymers. This conductivity can be adjusted by manipulating the element embedded in the cellulose fibers, depending on the application for which the paper is designed.

Thus, the device can be electrically conductive, allowing current to flow without significant losses, or semiconductive, interacting with specific molecules and functioning as a physical, chemical, or electrochemical sensor.

The study, by de Oliveira RF, was published in the April 2016 issue of the journal Organic Electronics.

Related Links:
Brazil National Nanotechnology Laboratory