Nucleic Acid Extracted from Clinical, Research, and Environmental Samples

The new E-Sphere Simple NA Kit was developed by Phthisis Diagnostics (Charlottesville, VA, USA). The E-Sphere Simple NA Kit is the first total nucleic acid extraction product in Phthisis Diagnostics’ Simply Molecular product line.

It develops enzyme-based extraction kits for rapid sample preparation prior to polymerase chain reaction (PCR) applications in laboratories. The kit’s optimized workflow minimizes procedural steps, sample transfers, and potential for error.

The novel E-Sphere method allows manual DNA/RNA extraction from 12 samples in less than 30 minutes total time–from sample to PCR. This streamlined process includes only 4 steps and minimizes contamination risk with only 1 to 2 sample transfers. Samples can be quickly processed within a biological safety cabinet.

Advanced technology in the kit includes the high performance E-Sphere enzyme, a thermostable reagent that digests the sample at 75 °C during 15-minute incubation. A further innovation is the proprietary E-Sphere Clean-up Column, which purifies the DNA/RNA for downstream analysis.

Laboratories have expressed their need for a faster, simpler method to purify DNA/RNA from difficult sample types prior to PCR amplification and analysis. This new kit answers the market need for speed among manual extraction methods and provides the added benefits of accuracy, improved cost effectiveness, reduced risk of sample contamination, and greater safety for lab technicians. The process requires only standard clinical laboratory equipment.

The new product is part of the developing Simply Molecular product catalog, a range of molecular diagnostic products designed for speed, accuracy, and ease of use that offer solutions for nucleic acid extraction, molecular diagnostics, and laboratory quality control.

Upcoming products will include molecular diagnostics for parasites and fungi, as well as molecular standards for improved laboratory quality control. Phthisis Diagnostics is also developing molecular diagnostic assays using polymerase chain reaction (PCR) technology to detect infectious diseases, including Cryptosporidium, Giardia, Microsporidia, and others.

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