Fully Automated Platform Designed for High-Throughput Genomic Cytometric Screening

The automated system, as configured, performs a diverse set of tasks important for carrying out functional genomics screens (cell culture, transfections, infections, sample concentration, and high-throughput flow cytometry) with very good throughput and minimal manual manipulation.

The technical poster presented at Society of Biomolecular Sciences Conference (SBS 2009) in April 2009 in Lille, France, showed how a proof-of-principle functional genomics shRNAmir screen for inhibitors of TGF-ß1 signaling in MPC9A7 cells identified several important regulators of this pathway. shRNAmirs targeting known signaling proteins such as Alk5 were readily detected using the HTFC capabilities of this platform.

With the automation configuration presented in the technical poster, throughput of the HyperCyt HTFC platform was doubled compared to stand alone operation. Data analysis software associated with the Agilent BioCel 1200 allowed plate level statistics to be generated within minutes of a screening run. In addition to speed, the technology is shown to considerably reduce the amount of sample aspirated for each analysis, typically acquiring two µL per well with thousands to tens of thousands of cells in each sample.

From standalone units to fully integrated systems, Agilent Automation Solutions combines key sample preparation and creative walk-away automation approaches with personalized customer service to provide complete solutions for laboratories.

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