We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Elevated Antibody Levels Identified in Chronic Fatigue Syndrome Patients

By LabMedica International staff writers
Posted on 28 Sep 2022
Print article
Image: Photomicrograph of the bacterium, Bacteroides hypermegas after being cultured in a thioglycollate medium, for a 48 hour time period. Members of the genus Bacteroides are anaerobic, Gram-negative bacteria, which are mainly found in the intestine, as normal flora (Photo courtesy of Dr. V. R. Dowell, Jr.)
Image: Photomicrograph of the bacterium, Bacteroides hypermegas after being cultured in a thioglycollate medium, for a 48 hour time period. Members of the genus Bacteroides are anaerobic, Gram-negative bacteria, which are mainly found in the intestine, as normal flora (Photo courtesy of Dr. V. R. Dowell, Jr.)

Patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) experience a lingering fatigue that can drastically impair their social and work life. ME/CFS affects approximately 1% of the population, appearing as a heterogeneous disease, with its etiology and pathogenesis still remaining elusive.

Given the diverse set of symptoms experienced by patients with severe ME/CFS beyond a debilitating fatigue, including sleep problems, muscle/joint pains, sore throat, and digestive issues such as irritable bowel syndrome, diagnosis is typically achieved by questionnaires rather than molecular markers.

A team of scientists from the Weizmann Institute of Science (Rehovot, Israel) used phage immunoprecipitation sequencing (PhIP-seq) to profile antibody responses of 40 patients with severe ME/CFS, as well as 40 healthy controls, against a library of 244,000 bacterial and viral epitopes. Serum samples of the severe ME/CFS cases and the healthy controls were obtained from the UKMEB. The antigen library encompasses diverse bacterial and viral antigens originating from pathogenic, probiotic, and commensal bacteria, including antigens selected from metagenomic sequencing.

The team reported that the ME/CFS patients exhibited high serum levels of immunoglobulin A against flagellins of Lachnospiraceae bacteria compared with the controls. Notably, a similar overrepresentation of antibodies against Lachnospiraceae flagellins has been reported in Crohn's disease, a common comorbidity in patients with severe ME/CFS. The scientists also identified lower levels of antibodies against Bacteroidetes in ME/CFS patients versus controls. The team used machine learning to determine how screening for antibodies against Lachnospiraceae could be used to diagnose ME/CFS and found that combining conventional blood tests for the disorder with antibody repertoire data significantly improved diagnosis.

The authors concluded that in addition to their diagnostic implications, the findings also may help in the development of therapeutic strategies for ME/CFS that target the identified antibody responses. Such efforts may include preventive vaccination with Lachnospiraceae or Bacteroidetes surface antigens to counteract anti-flagellin overreaction, as well as therapies reducing anti-flagellin binding. The study was published on September 23, 2022 in the journal Science Advances.

Related Links:
Weizmann Institute of Science

Gold Supplier
Blood Glucose Reference Analyzer
Nova Primary
Thyroglobulin (Tg) Assay
Gold Supplier
Virus Detection System
Radiotherapy Toxicity Measurement Assay
Radiotherapy Toxicity Measurement Assay

Print article


Clinical Chem.

view channel
Image: Brief schematic diagram of the detection principle and method (Photo courtesy of CAS)

Rapid, Non-Invasive Method Diagnoses Type 2 Diabetes by Sniffing Urinary Acetone

Over 90% of diabetes cases are attributed to Type 2 diabetes (T2D), a prevalent metabolic condition that is expected to impact 380 million individuals globally by 2025. Despite being highly accurate, the... Read more

Molecular Diagnostics

view channel
Image: Researchers have identified the origin of subset of autoantibodies that worsen lupus (Photo courtesy of Pexels)

Lupus Biomarker Testing Could Help Identify Patients That Need Early and Aggressive Treatment

Systemic lupus erythematosus (SLE) is an autoimmune disease that occurs when the body's antibodies, which usually protect against infections, attack healthy cells and proteins. These autoantibodies can... Read more


view channel
Image: Use of DBS samples can break barriers in hepatitis C diagnosis and treatment for populations at risk (Photo courtesy of Pexels)

DBS-Based Assay Effective in Hepatitis C Diagnosis and Treatment for At Risk Populations

In a bid to eliminate viral hepatitis as a public health threat by 2030, the World Health Organization (WHO) has put forth a proposed strategy. To this end, researchers at the Germans Trias i Pujol Research... Read more


view channel
Image: New research has opened a path for fast and accurate cancer diagnosis (Photo courtesy of Imagene)

AI-Based Image Analysis Software Profiles Cancer Biomarkers in Real Time

Lung cancer is the most widespread type of cancer worldwide, resulting in approximately 1.76 million fatalities annually. Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancer diagnoses... Read more


view channel
Image: Live view of non-fluorescent specimens using the glowscope frame (Photo courtesy of Winona State University)

Device Converts Smartphone into Fluorescence Microscope for Just USD 50

Fluorescence microscopes are utilized to examine specimens labeled with fluorescent stains or expressing fluorescent proteins, like those tagged with green fluorescent protein. However, since these microscopes... Read more


view channel
Image: The global antimicrobial resistance diagnostics market size is expected to reach USD 5.7 billion by 2028 (Photo courtesy of Pexels)

Global Antimicrobial Resistance Diagnostics Market Driven by Increasing Hospital-Acquired Infections

Antimicrobial drugs are intended to counteract the harmful effects of microbes and promote a healthy life. However, their excessive use can result in the development of resistance, commonly referred to... Read more
Copyright © 2000-2023 Globetech Media. All rights reserved.