We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Microbiome and Metabolome Features Characterized for the Cardiometabolic Disease Spectrum

By LabMedica International staff writers
Posted on 28 Feb 2022
Print article
Image: The BD Accuri C6 Plus Flow Cytometer (Photo courtesy of BD Biosciences)
Image: The BD Accuri C6 Plus Flow Cytometer (Photo courtesy of BD Biosciences)

Scientists have already discovered that the gut microbiome is altered in people with chronic heart disease. They subsequently identified compounds that are produced by the diseased microbiome, for instance a bacterial compound called trimethylamine (TMA) that after modification in the liver of the human host causes arteriosclerosis.

The human gut contains trillions of bacteria, collectively called the gut microbiome, which may have positive and negative effects on human health. When in balance they function as an inner chemistry factory producing numerous compounds that promote good health.

However, an unhealthy lifestyle can disrupt the balance, leading the microbiome to instead produce compounds that may trigger multiple non-communicable chronic disorders in people at high genetic risk, including myocardial infarction, angina or heart failure. Scientists have already discovered that the gut microbiome is altered in people with chronic heart disease.

A large team of international scientists including those at the University of Copenhagen (Copenhagen, Denmark) recruited 1,241 middle-aged people from Denmark, France and Germany including healthy individuals, individuals with obesity and type 2 diabetes, but lacking a diagnosis of heart disease, and patients with either myocardial infarction, angina pectoris or heart failure. Fasting plasma glucose, total and HDL cholesterol, triglycerides, creatinine and HbA1c levels were measured using standard enzymatic methods.

Alanine aminotransferase, aspartate aminotransferase and γ-glutamyltransferase were measured by enzyme-coupled kinetic assays. Ultra-sensitive C-reactive protein was measured using an Image Automatic Immunoassay System (Beckman Coulter, Brea, CA. USA). Microbial loads of fecal samples were processed and analyzed using a C6 Accuri flow cytometer (BD Biosciences, San Diego, CA, USA).

The scientists found that about 75% of microbiome and metabolome features that distinguish individuals with ischemic heart disease (IHD) from healthy individuals after adjustment for effects of medication and lifestyle are present in individuals exhibiting dysmetabolism, suggesting that major alterations of the gut microbiome and metabolome might begin long before clinical onset of IHD. They further categorized microbiome and metabolome signatures related to prodromal dysmetabolism; specific to IHD in general or to each of its three subtypes or related to escalation or de-escalation of IHD. At higher microbiome architecture levels, there was a significant shift from the Bacteroides 1 and Ruminococcus enterotypes toward the low bacterial cell-count-associated Bacteroides 2 as the disease worsened.

Oluf Borbye Pedersen, MD, A Clinical Professor and a senior author of the study, said, “We found that about half of these gut bacteria and blood compounds were modified by drug treatment and not directly related to heart disease or the early disease stages like diabetes or obesity occurring prior to diagnosis of heart disease. Among the remaining half, about 75% of the disturbances of the gut microbiome occurred in the early disease stages of overweight and type 2 diabetes, many years before patients noticed any symptoms of heart disease.”

The authors concluded that at prodromal dysmetabolic stages and at both early and late clinical manifestations of IHD, multiple de-confounded microbiome and metabolome alterations are present, reflecting distinct metabolic pathways. Several of these are modifiable and might be targets for future mechanistic studies and clinical trials aiming at IHD prevention. The study was published on February 17, 2022 in the journal Nature Medicine.

Related Links:
University of Copenhagen 
Beckman Coulter
BD Biosciences 

Gold Supplier
Microscopes and Accessories
Fecal Calprotectin Testing Reagent
OC-FCa Reagent
Clostridium Difficile Test
Simplexa C. difficile Direct Kit
Squamous Cell Carcinoma Assay

Print article


Clinical Chem.

view channel
Image: Brief schematic diagram of the detection principle and method (Photo courtesy of CAS)

Rapid, Non-Invasive Method Diagnoses Type 2 Diabetes by Sniffing Urinary Acetone

Over 90% of diabetes cases are attributed to Type 2 diabetes (T2D), a prevalent metabolic condition that is expected to impact 380 million individuals globally by 2025. Despite being highly accurate, the... Read more

Molecular Diagnostics

view channel
Image: Researchers have identified the origin of subset of autoantibodies that worsen lupus (Photo courtesy of Pexels)

Lupus Biomarker Testing Could Help Identify Patients That Need Early and Aggressive Treatment

Systemic lupus erythematosus (SLE) is an autoimmune disease that occurs when the body's antibodies, which usually protect against infections, attack healthy cells and proteins. These autoantibodies can... Read more


view channel
Image: A genetic test could guide the use of cancer chemotherapy (Photo courtesy of Pexels)

Genetic Test Predicts Whether Bowel Cancer Patients Can Benefit From Chemotherapy

Late-stage bowel cancer patients usually undergo a series of chemotherapies and targeted medicines for cancer treatment. However, the responses to the last-line chemotherapy treatment trifluridine/tipiracil... Read more


view channel
Image: New research has opened a path for fast and accurate cancer diagnosis (Photo courtesy of Imagene)

AI-Based Image Analysis Software Profiles Cancer Biomarkers in Real Time

Lung cancer is the most widespread type of cancer worldwide, resulting in approximately 1.76 million fatalities annually. Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancer diagnoses... Read more


view channel
Image: Live view of non-fluorescent specimens using the glowscope frame (Photo courtesy of Winona State University)

Device Converts Smartphone into Fluorescence Microscope for Just USD 50

Fluorescence microscopes are utilized to examine specimens labeled with fluorescent stains or expressing fluorescent proteins, like those tagged with green fluorescent protein. However, since these microscopes... Read more


view channel
Image: The global antimicrobial resistance diagnostics market size is expected to reach USD 5.7 billion by 2028 (Photo courtesy of Pexels)

Global Antimicrobial Resistance Diagnostics Market Driven by Increasing Hospital-Acquired Infections

Antimicrobial drugs are intended to counteract the harmful effects of microbes and promote a healthy life. However, their excessive use can result in the development of resistance, commonly referred to... Read more
Copyright © 2000-2023 Globetech Media. All rights reserved.