We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Luminex Corporation

Luminex develops, manufactures, and markets biological testing technologies with applications in clinical diagnostics... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
20 Oct 2021 - 22 Oct 2021
21 Oct 2021 - 24 Oct 2021
Virtual Venue

Biological Markers Associated with Severe Dengue Identified

By LabMedica International staff writers
Posted on 06 Jul 2021
Print article
Image: The Luminex200 Instrument System sets the standard for multiplexing, providing the ability to perform up to 100 different tests in a single reaction volume on a flow cytometry-based platform (Photo courts of Luminex Corporation)
Image: The Luminex200 Instrument System sets the standard for multiplexing, providing the ability to perform up to 100 different tests in a single reaction volume on a flow cytometry-based platform (Photo courts of Luminex Corporation)
Dengue is the most common mosquito-borne viral disease to affect humans globally. In 2019, the World Health Organization identified dengue as one of the top 10 threats to global health, with transmission occurring in 129 countries and an estimated 3.9 billion people being at risk.

Biomarkers are used to identify the state or risk of a disease in patients. Examples of biomarkers can include naturally occurring molecules or genes in the vascular, inflammatory or other biological pathways. New findings could aid the development of biomarker panels for clinical use and help improve triage and risk prediction in patients with dengue.

A large team of international scientists led by the Oxford University Clinical Research Unit (OUCRU, Ho Chi Minh City, Viet Nam) conducted a study using samples and clinical information from a large multi-country observational study. Of the 2,694 laboratory-confirmed dengue cases included in the study, 38 and 266 cases were classified as severe and moderate dengue, respectively. The team selected 281 cases in four countries, Vietnam, Cambodia, Malaysia and El Salvador, as the blood samples from these participants were stored at the OUCRU laboratory. For comparison, the team also selected 556 patients with uncomplicated dengue who shared similar geographies and demographic characteristics.

The biomarkers were measured at two time points: at enrollment (illness day 1-3) and after recovery (day 10-31 post-symptom onset), if available. Eight biomarkers: VCAM-1, SDC-1, Ang-2, IL-8, IP-10, IL-1RA, sCD163, sTREM-1, were combined in a premixed magnetic bead panel (R&D Systems, Minneapolis, MN, USA). C-reactive protein (CRP) was measured using a separate commercial magnetic bead panel (EMD Millipore Corporation, Burlington, MA, USA). These panels were analyzed using the Luminex200 analyzer with the Luminex calibration (Austin, TX, USA). Ferritin was measured using the Human Ferritin ELISA kit (Arigo Biolaboratories, Hsinchu City, Taiwan, ROC).

The investigators reported that on days 1-3 from symptom onset, higher levels of any biomarker increased the risk of developing severe and moderate dengue (S/MD). When assessing together, SDC-1 and IL-1RA were stable, while IP-10 changed the association from positive to negative; others showed weaker associations. The best combinations associated with S/MD comprised IL-1RA, Ang-2, IL-8, ferritin, IP-10, and SDC-1 for children, and SDC-1, IL-8, ferritin, sTREM-1, IL-1RA, IP-10, and sCD163 for adults. They found that, during the first three days of illness, higher levels of any of the 10 biomarkers increased a patient's risk of developing moderate to severe dengue.

Sophie Yacoub, PhD, a Consultant Physician and a senior author of the study, said, “Together, our findings should assist the development of biomarker panels to help improve future triage and early assessment of dengue patients. This would help improve individual patient management and healthcare allocation, which would be of major public health benefit especially in outbreak settings.”

The authors concluded that higher levels of the ten biomarkers (VCAM-1, SDC-1, Ang-2, IL-8, IP-10, IL-1RA, sCD163, sTREM-1, ferritin, and CRP), when considered individually, are associated with increased risk of adverse clinical outcomes in both children and adults with dengue. The study was published on June 22, 2021 in the journal eLife.

Related Links:
Oxford University Clinical Research Unit
R&D Systems
EMD Millipore Corporation
Luminex Corporation
Arigo Biolaboratories


New
Gold Supplier
Molecular Diagnostic System
Singuway 9600 Pro
New
Microplate Reader
Sunrise
New
Molecular Diagnostic Platform
VERIGENE II System
New
ELISA Mini Workstation
Crocodile 5-in-one

Print article

Channels

Molecular Diagnostics

view channel
Image: The QuikRead go iFOBT is an immunochemical fecal immunochemical test for detection and quantification of human hemoglobin in feces in case of suspected bleeding from the lower gastrointestinal tract (Photo courtesy of Aidian)

Fecal Immunochemical Tubes Sourced to Analyze Gut Microbiome for CRC

Colorectal cancer (CRC) is a challenging public health problem which successful treatment depends on the stage at diagnosis. Recently, CRC-specific microbiome signatures have been proposed as a marker... Read more

Hematology

view channel
Image: Bone marrow aspirate from a patient with peripheral T-cell lymphoma (Photo courtesy of Peter Maslak, MD)

Mutation Analysis Links Angioimmunoblastic T-Cell Lymphoma to Clonal Hematopoiesis

Peripheral T-cell lymphoma (PTCL) is a heterogeneous group of lymphoid tumors and encompass peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS), angioimmunoblastic T-cell lymphoma (AITL), and... Read more

Industry

view channel
Image: GastroPanel Quick Test (Photo courtesy of Biohit Healthcare)

Biohit’s Innovative GastroPanel Quick Test Receives CE Mark

Biohit Healthcare’s (Helsinki, Finland) GastroPanel Quick Test, the latest innovation in its unique GastroPanel product family, is now CE marked. The GastroPanel Quick Test is intended for diagnosing... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.